Interaction and effectiveness of antimicrobials along with healing-promoting agents in a novel biocellulose wound dressing
Interaction and effectiveness of antimicrobials along with healing-promoting agents in a novel biocellulose wound dressing
复制标题
DOI：
10.1016/j.msec.2015.05.026
复制
发表时间：
2015-10-01
影响因子：
7.9
通讯作者：
Aramwit, Pornanong
中科院分区：
文献类型：
--
作者：
Napavichayanun, Supamas;Amornsudthiwat, Phakdee;Aramwit, Pornanong
理想的伤口敷料应保持伤口湿润，允许氧气渗透，吸附伤口渗出物，加速伤口闭合的上皮再生，减少疼痛和愈合时间，并预防感染。我们的新型生物纤维素基伤口敷料由三种成分组成：1）生物纤维素（BC），旨在创造一个潮湿和透氧的环境，并具有渗出物吸附作用; 2）蚕丝丝胶蛋白（SS），以其增强I型胶原蛋白的产生而闻名，这对上皮再生至关重要; 3）防腐剂聚乙二醇双胍（PHMB）。为了提供有效的BC伤口敷料，需要彻底分析组分（PHMB与SS）之间的相互作用。在本研究中，我们研究了活性化合物的加载顺序、加载浓度和加载量等重要参数，以确保BC伤口敷料在愈合过程中既能提供抗菌活性，又能促进胶原蛋白的产生。将SS和PHMB加载到BC中的顺序对于保持PHMB抗菌活性是至关重要的;丝丝胶蛋白需要在PHMB之前加载以避免任何负面影响。有效消除所有测试细菌（枯草芽孢杆菌、金黄色葡萄球菌、耐甲氧西林S.金黄色葡萄球菌、大肠杆菌、鲍曼不动杆菌和铜绿假单胞菌）。优化BC中SS和PHMB的量，以确保敷料释放最佳量的SS，以增强成纤维细胞胶原蛋白的产生，并释放最佳量的PHMB，以获得有效的抗菌活性。(C)2015 Elsevier B. V.版权所有。
An ideal wound dressing should keep the wound moist, allow oxygen permeation, adsorb wound exudate, accelerate re-epithelialization for wound closure, reduce pain and healing time, and prevent infection. Our novel biocellulose-based wound dressing was composed of three components: 1) biocellulose (BC), intended to create a moist and oxygen-permeated environment with exudate adsorption; 2) silk sericin (SS) known for its enhancement of collagen type I production, which is critical for re-epithelialization; and 3) the antiseptic polyhexamethylene biguanide (PHMB). To deliver an effective BC wound dressing, the interactions between the components (PHMB vs. SS) needed to be thoroughly analyzed. In this study, we investigated important parameters such as the loading sequence, loading concentration, and loading amount of the active compounds to ensure that the BC wound dressing could provide both antimicrobial activity and promote collagen production during healing. The loading sequence of SS and PHMB into BC was critical to maintain PHMB antimicrobial activity; silk sericin needed to be loaded before PHMB to avoid any negative impacts. The minimum PHMB concentration was 0.3% w/v for effective elimination of all tested bacteria (Bacillus subtilis, Staphylococcus aureus, methicillin-resistant S. aureus, Fscherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa). The amounts of SS and PHMB in BC were optimized to ensure that the dressings released the optimal amounts of both SS to enhance fibroblast collagen production and PHMB for effective antimicrobial activity. (C) 2015 Elsevier B.V. All rights reserved.