Labeling of skeletal myoblasts with a novel oxygen-sensing spin probe for noninvasive monitoring of in situ oxygenation and cell therapy in heart.
Labeling of skeletal myoblasts with a novel oxygen-sensing spin probe for noninvasive monitoring of in situ oxygenation and cell therapy in heart.
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用新型氧传感旋转探针标记骨骼肌母细胞，用于无创监测心脏原位氧合和细胞治疗。
DOI：
10.1152/ajpheart.01058.2006
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Kuppusamy,Periannan
中科院分区：
文献类型：
--
作者：
Wisel,Sheik;Chacko,SimiM;Kuppusamy,MLakshmi;Pandian,RamasamyP;Khan,Mahmood;Kutala,VijayKumar;Burry,RichardW;Sun,Benjamin;Kwiatkowski,Pawel;Kuppusamy,Periannan
我们报告了用一种新型氧传感顺磁自旋探针对骨骼肌母细胞（SM）进行标记（内化），用于使用电子顺磁共振（EPR）光谱对干细胞治疗中的氧合进行无创跟踪和原位监测。 SM细胞从小鼠大腿肌肉活检中分离出来并在培养物中增殖。 SM 细胞与探针的标记是通过将细胞与亚微米大小 (270 ± 120 nm) 的探针颗粒共孵育 48 小时来实现的。标记对细胞的活力或增殖没有显着影响。将探针标记的 SM 细胞移植到小鼠心脏的梗塞区域。通过使用骨骼肌细胞的 MY-32 染色来验证移植细胞在梗塞区域的植入。移植后4周内重复无创地测定心脏原位Po2。结果显示，与未治疗的对照相比，细胞移植部位的心肌氧合显着增强。总之，用氧传感旋转探针标记 SM 细胞为移植细胞以及梗塞小鼠心脏原位组织 Po2 的无创监测提供了独特的机会。
We report the labeling (internalization) of skeletal myoblasts (SMs) with a novel class of oxygen-sensing paramagnetic spin probe for noninvasive tracking and in situ monitoring of oxygenation in stem cell therapy using electron paramagnetic resonance (EPR) spectroscopy. SM cells were isolated from thigh muscle biopsies of mice and propagated in culture. Labeling of SM cells with the probe was achieved by coincubating the cells with submicron-sized (270 ± 120 nm) particulates of the probe in culture for 48 h. The labeling had no significant effect on the viability or proliferation of the cells. The SM cells labeled with the probe were transplanted in the infarcted region of mouse hearts. The engraftment of the transplanted cells in the infarct region was verified by using MY-32 staining for skeletal myocytes. The in situ Po2in the heart was determined noninvasively and repeatedly for 4 wk after transplantation. The results showed significant enhancement of myocardial oxygenation at the site of cell transplant compared with untreated control. In conclusion, labeling of SM cells with the oxygen-sensing spin probe offers a unique opportunity for the noninvasive monitoring of transplanted cells as well as in situ tissue Po2in infarcted mouse hearts.