Developmental regulation of nodule-specific genes in alfalfa root nodules.
Developmental regulation of nodule-specific genes in alfalfa root nodules.
复制标题
苜蓿根瘤中根瘤特异性基因的发育调控。
DOI：
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发表时间：
1988
影响因子：
3.5
通讯作者：
F. Ausubel
中科院分区：
文献类型：
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作者：
K. Dunn;R. Dickstein;Rhonda L. Feinbaum;Burnett Bk;T. Peterman;G. Thoidis;H. Goodman;F. Ausubel
我们克隆了苜蓿结节特异性cdna，编码豆血红蛋白（Lb）、谷氨酰胺合成酶（GS）和三种未识别的结节蛋白。对结核RNA进行杂交选择翻译，然后进行二维凝胶电泳，结果表明，Lb特异性cDNA至少对应4个Lb物种，长度为12 kDa。其中一个未确定的cDNA克隆（N-32/34）至少对应5个32-34 kDa的多肽；另一个未识别的cDNA克隆（N-14）对应于一个14 kDa的单个多肽。第三个cDNA克隆（N-22）选择的RNA杂交体外翻译产物(s)在一维凝胶上形成22 kDa的单带。对野生型根瘤和多种根瘤菌突变体诱导的缺陷根瘤的RNA进行Northern和斑点杂交分析，结果表明：1)在根瘤发育过程中，Lb、根瘤特异性GS和3种未知根瘤蛋白对应的RNA协同表达；2) 5种结核蛋白均在含有感染线和类杆菌的固定型结核中表达，而在缺乏感染线和细胞内根瘤菌的固定型结核中不表达。
We have cloned alfalfa nodule-specific cDNAs that code for leghemoglobin (Lb), glutamine synthetase (GS), and three unidentified nodulins. Hybrid-select translation of nodule RNA followed by 2-D gel electrophoresis showed that the Lb-specific cDNA corresponded to at least four Lb species of 12 kDa. One of the unidentified cDNA clones (N-32/34) corresponded to at least five polypeptides of 32-34 kDa; a second unidentified cDNA clone (N-14) corresponded to an individual polypeptide of 14 kDa. The in vitro translation product(s) of the RNA hybrid selected by the third unidentified cDNA clone (N-22) formed a single band at 22 kDa on a one-dimensional gel. Northern and dot blot analyses of RNA isolated from wild-type nodules and from defective nodules elicited by a variety of Rhizobium meliloti mutants showed that 1) RNAs corresponding to the Lb, nodule-specific GS, and three unidentified nodulins were coordinately expressed during the course of nodule development, and 2) all five nodulins were expressed in Fix- nodules that contained infection threads and bacteroids but were not expressed in nodules that lacked infection threads and intracellular rhizobia.