Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus.
Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus.
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DOI：
10.1093/gbe/evx037
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发表时间：
2017-04-01
影响因子：
3.3
通讯作者：
Sheppard SK
中科院分区：
文献类型：
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作者：
Murray S;Pascoe B;Méric G;Mageiros L;Yahara K;Hitchings MD;Friedmann Y;Wilkinson TS;Gormley FJ;Mack D;Bray JE;Lamble S;Bowden R;Jolley KA;Maiden MCJ;Wendlandt S;Schwarz S;Corander J;Fitzgerald JR;Sheppard SK
金黄色葡萄球菌在养殖的鸡群中广泛传播，引起骨骼肌感染、皮炎和败血症。家禽相关谱系的出现涉及从人到鸡的人畜共患病传播，但关于促进鸡病原体增殖的特定适应的问题仍然存在。我们在一个基因组测序的S。来源于家禽和人类的金黄色葡萄球菌分离株。系谱分析确定了一个占主导地位的家禽相关序列簇内的CC5克隆复合物。禽和人CC5分离株彼此显著不同，并且在禽分离株中检测到更多的重组事件。我们确定了沿着延伸到家禽特异性CC5簇的分支的33个基因中的44个重组事件，并且与来自人类的那些相比，在CC5家禽分离株中更常见地发现了47个基因。这些基因序列中的许多是常见的鸡分离物从其他克隆复合体表明家禽相关谱系之间的水平基因转移。与推定的家禽相关基因的功能预测一致，与人类分离株相比，家禽分离株在42 °C下显示出增强的生长，并且在鸡血琼脂上显示出更大的红细胞溶解。通过结合表型信息与葡萄球菌基因组的进化分析，我们提供了适应的证据，人类到家禽的宿主过渡。这对与现代农业有关的新的致病性克隆的出现和传播具有重要意义。
Staphylococcus aureus are globally disseminated among farmed chickens causing skeletal muscle infections, dermatitis, and septicaemia. The emergence of poultry-associated lineages has involved zoonotic transmission from humans to chickens but questions remain about the specific adaptations that promote proliferation of chicken pathogens. We characterized genetic variation in a population of genome-sequenced S. aureus isolates of poultry and human origin. Genealogical analysis identified a dominant poultry-associated sequence cluster within the CC5 clonal complex. Poultry and human CC5 isolates were significantly distinct from each other and more recombination events were detected in the poultry isolates. We identified 44 recombination events in 33 genes along the branch extending to the poultry-specific CC5 cluster, and 47 genes were found more often in CC5 poultry isolates compared with those from humans. Many of these gene sequences were common in chicken isolates from other clonal complexes suggesting horizontal gene transfer among poultry associated lineages. Consistent with functional predictions for putative poultry-associated genes, poultry isolates showed enhanced growth at 42 °C and greater erythrocyte lysis on chicken blood agar in comparison with human isolates. By combining phenotype information with evolutionary analyses of staphylococcal genomes, we provide evidence of adaptation, following a human-to-poultry host transition. This has important implications for the emergence and dissemination of new pathogenic clones associated with modern agriculture.