Establishment of PLAFMCi007-A, an induced pluripotent stem cell line, from peripheral blood mononuclear cells (PBMCs) of a healthy adult woman.
Establishment of PLAFMCi007-A, an induced pluripotent stem cell line, from peripheral blood mononuclear cells (PBMCs) of a healthy adult woman.
复制标题
从健康成年女性的外周血单核细胞 (PBMC) 中建立 PLAFMCi007-A，这是一种诱导多能干细胞系。
DOI：
10.1016/j.scr.2022.102760
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发表时间：
2022
影响因子：
1.2
通讯作者：
Xueyuan Bai
中科院分区：
文献类型：
--
作者：
Meihan Shi;Shunlai Shang;Yunzhao Yang;Qinggang Li;Xueyuan Bai
Induced pluripotent stem cell (iPSC) lines for studies investigating many diseases can be established from peripheral blood mononuclear cells; here, an iPSC line was established from CD34+cells isolated from the peripheral blood of a healthy woman. The cells were electrotransfected with three different recombinant plasmids to generate a normal-karyotype iPSC line that expresses characteristic surface markers and other pluripotent stem cell genes and can differentiate into all three germ layers in vivo. These newly established iPSC lines, a normal human cell line, can serve as a control line in studies investigating the pathogenesis of various diseases and meet the conditions for organoid studies.
