Polydopamine-mediated preparation of an enzyme-immobilized microreactor for the rapid production of wax ester
Polydopamine-mediated preparation of an enzyme-immobilized microreactor for the rapid production of wax ester
复制标题
聚多巴胺介导的酶固定微反应器的制备用于快速生产蜡酯
DOI：
10.1039/c7ra00499k
复制
发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Wei, Ping
中科院分区：
文献类型：
--
作者：
Bi, Yicheng;Zhou, Hua;Wei, Ping
本研究采用多巴胺聚合和多层沉积的方法，成功制备了聚四氟乙烯（PTFE）开管式固定化酶微反应器。聚多巴胺层被用作微通道中的底漆层以提供PTFE表面可反应基团，并且聚乙烯亚胺和Candida anastritica脂肪酶B通过逐层方法交替吸附在PTFE表面上。脂肪酶的负载量随着层数的增加而增加，在第8层达到稳定。通过水接触角测量、傅里叶变换红外光谱（FI-IR）和X射线光电子能谱（XPS）对多层膜的形成和酶负载量的增加进行了描述和解释，并通过原子力显微镜（AFM）和场发射扫描电子显微镜（FE-SEM）观察了其形态特征。使用微反应器制备蜡酯的生产效率（35分钟达到95%产率）比使用传统反应器（超过4小时完成反应）好得多。该微反应器具有很好的稳定性，可使用144小时，剩余活性保持在初始活性的83%。
In our study, a polytetrafluoroethylene (PTFE) open-tubular enzyme-immobilized microreactor has been successfully prepared using dopamine polymerization and multi-layer deposition. The polydopamine layer was utilized as a primer layer in the microchannel to provide PTFE surface reactable groups, and polyethylenimine and Candida antarctica lipase B were alternatively adsorbed on the PTFE surface by the layer-by-layer method. The lipase loading kept increasing as the number of layers increased and reached a plateau at the 8th layer. The formation of multilayers and increase of enzyme loading were described and explained by the water contact angle measurement, Fourier transform infrared spectroscopy (FI-IR), and X-ray photoelectron spectroscopy, while the morphological characteristics were observed using Atomic Force Microscopy (AFM) and field emission scanning electron microscopy (FE-SEM). Preparation of a wax ester using the microreactor had much better production efficiency (35 minutes to reach 95% yield) than that using traditional reactors (over 4 hours to complete the reaction). Very excellent stability can be achieved by the microreactor, which could be used for 144 hours with the residual activity remaining at 83% of the initial activity.