Short collagen fibers provide control of contraction and permeability in fibroblast-seeded collagen gels
Short collagen fibers provide control of contraction and permeability in fibroblast-seeded collagen gels
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DOI：
10.1089/107632704323061780
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发表时间：
2004-03-01
影响因子：
--
通讯作者：
Livesay, GA
中科院分区：
文献类型：
--
作者：
Gentleman, E;Nauman, EA;Livesay, GA
组织工程可以允许重建人体中的乳房、面部、皮肤和其他软组织缺损。细胞接种的胶原蛋白凝胶是创造软组织的合理选择，因为它们是可生物降解的，模仿天然组织，并为细胞提供三维环境。然而，与这种方法相关的主要缺点是凝胶随后被组成细胞收缩，这严重降低了渗透性，引发了细胞凋亡，并妨碍了对所得到的构建体的形状和大小的控制。在这项研究中，I型胶原凝胶接种成纤维细胞和铸造或添加或不添加短胶原纤维。在培养7天和14天后监测凝胶收缩并评估渗透性。短胶原纤维的加入既显著限制了成纤维细胞接种的胶原凝胶的收缩又增加了其渗透性。短胶原纤维的加入对细胞增殖没有不利影响，并且在具有纤维的凝胶和不具有纤维的凝胶中有大量的活成纤维细胞。含有短胶原纤维的凝胶表现出比对照高100至1000倍的渗透性，并且还紧密地保持其铸件尺寸（从不低于原始尺寸的96%）。通过限制收缩和保持渗透性，短胶原纤维的掺入应该能够通过允许更大的营养扩散来创建更大的构造，并且允许在凝胶浇铸期间创建更复杂的形状。
Tissue engineering may allow for the reconstruction of breast, facial, skin, and other soft tissue defects in the human body. Cell-seeded collagen gels are a logical choice for creating soft tissues because they are biodegradable, mimic the natural tissue, and provide a three-dimensional environment for the cells. The main drawback associated with this approach, however, is the subsequent contraction of the gel by the constituent cells, which severely reduces permeability, initiates apoptosis, and precludes control of the resulting shape and size of the construct. In this study, type I collagen gels were seeded with fibroblasts and cast either with or without the addition of short collagen fibers. Gel contraction was monitored and permeability was assessed after 7 and 14 days in culture. The addition of short collagen fibers both significantly limited contraction and increased permeability of fibroblast-seeded collagen gels. The addition of short collagen fibers had no detrimental effect on cell proliferation, and there were a high number of viable fibroblasts in gels with fibers and gels without fibers. Gels containing short collagen fibers demonstrated permeabilities that were 100 to 1000 times greater than controls and also closely maintained their casting dimensions (never less than 96% of original). By limiting contraction and maintaining permeability, the incorporation of short collagen fibers should enable the creation of larger constructs by allowing for greater nutrient diffusion, and permit the creation of more complicated shapes during gel casting.