Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source
Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source
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DOI：
10.1016/j.biortech.2013.04.124
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发表时间：
2013-08-01
影响因子：
11.4
通讯作者：
Ma, Yanhe
中科院分区：
文献类型：
--
作者：
Li, Yuan;Wang, Limin;Ma, Yanhe
在本研究中，我们发现Sporolacilluslaevolacticus DSM 442可以通过使用棉籽作为唯一的氮源来产生D-乳酸。以棉籽为原料，采用酶法水解并同时利用棉籽进行D-乳酸发酵。在最佳条件下，补料分批发酵35 h，D-乳酸浓度可达144.4g/L，平均生产力为4.13g/（Lh），产率为0.96g/g葡萄糖。发酵液中D-乳酸的光学纯度为99.3%，符合乳酸聚合的要求。我们的研究代表了一种使用廉价农业废物生产聚合物级D-乳酸盐的具有成本效益的方法。(C)2013爱思唯尔有限公司保留所有权利。
In this study, we show that Sporolactobacillus laevolacticus DSM442 can produce D-lactate by using cottonseed as the sole nitrogen source. The cottonseed was enzymatically hydrolyzed and simultaneously utilized during D-lactate fermentation. Under optimal conditions, a high D-lactic acid concentration (144.4 g/L) was obtained in a fed-batch fermentation within 35 h, with an average productivity of 4.13 g/(L h) and a yield of 0.96 g/g glucose. The optical purity of D-lactic acid in the broth was 99.3%, which meets the requirement for use in lactic acid polymerization. Our study represents a cost-effective method for polymer-grade D-lactate production using cheap agricultural wastes. (C) 2013 Elsevier Ltd. All rights reserved.