Cyclic nucleotide metabolism in compensatory renal hypertrophy and neonatal kidney growth.
Cyclic nucleotide metabolism in compensatory renal hypertrophy and neonatal kidney growth.
复制标题
代偿性肾肥大和新生儿肾脏生长中的环核苷酸代谢。
DOI：
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发表时间：
1976
影响因子：
11.1
通讯作者：
H. Weber
中科院分区：
文献类型：
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作者：
D. Schlondorff;H. Weber
研究了大鼠代偿性肥大和新生儿发育过程中生长期肾脏的环核苷酸代谢。在单侧肾切除术后，在肥大的肾脏中观察到环磷酸腺苷（cAMP）的轻度和短暂的减少。相反，环3 '：5'鸟苷酸（cGMP）在15分钟时急剧下降至对照的20%，在1-72小时时快速上升至基线以上的200-300%。肾组织cGMP水平的改变与可溶性100，000 X g上清液鸟苷酸环化酶活性的平行变化有关[GTP焦磷酸裂解酶（环化）：EC 4.6.1.2]。未观察到总cGMP磷酸二酯酶（3 '：5'-环核苷酸5 '-核苷酸水解酶; EC 3.1.4.17）的变化。在新生大鼠快速生长的肾脏中，cAMP水平在出生后4天和7天分别为983 +/- 65和833 +/- 42 pmol/g肾脏，并增加到成人水平在第21天，cGMP水平为1518 +/- 57 pmol/g，而在第4天和第7天，cGMP水平为59.8 +/- 6.8和92.5 +/- 13.9 pmol/g，并在第21天降至成人水平（36 +/- 1.5）。结果表明，代偿性肾脏肥大和新生肾脏生长与cAMP和cGMP代谢的变化有关。
Cyclic nucleotide metabolism was investigated in growing kidneys of rats during compensatory hypertrophy and during neonatal development. After unilateral nephrectomy a mild and short-lasting decrease in cyclic 3':5" adenosine monophosphate (cAMP) was observed in the hypertrophying kidney. In contrast, cyclic 3':5' guanosine monophosphate (cGMP) showed a sharp decline to 20% of control at 15 min and a rapid rise to 200-300% above base-line at 1-72 hr. The alterations in renal tissue levels of cGMP were associated with parallel changes in the soluble, 100,000 X g supernatant guanylate cyclase activity [GTP pyrophosphate-lyase (cyclizing): EC 4.6.1.2]. No change was observed in total cGMP phosphodiesterase (3':5'-cyclic-nucleotide 5'-nucleotidohydrolase; EC 3.1.4.17). In the rapidly growing kidney of newborn rats cAMP levels were 983 +/- 65 and 833 +/- 42 pmol/g of kidney at 4 and 7 days after birth, and increased to adult levels (1518 +/- 57 pmol/g) at 21 days whereas cGMP levels were 59.8 +/- 6.8 and 92.5 +/- 13.9 pmol/g at 4 and 7 days and decreased to adult levels (36 +/- 1.5) at 21 days. The results indicate that compensatory renal hypertrophy and neonatal kidney growth are associated with changes in cAMP and cGMP metabolism.