The key role of CYC2 during meiosis in Tetrahymena thermophila.
The key role of CYC2 during meiosis in Tetrahymena thermophila.
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DOI：
10.1007/s13238-016-0254-9
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发表时间：
2016-04
期刊：
影响因子：
21.1
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Xu Q;Wang R;Ghanam AR;Yan G;Miao W;Song X
减数分裂重组是通过Spo 11蛋白形成和修复DNA双链断裂（DSB）的专门途径进行的。本研究揭示了细胞周期蛋白CYC 2在嗜热四膜虫减数分裂过程中的功能作用。敲除CYC 2基因导致减数分裂接合过程在接合开始后2.5-3.5小时、减数分裂分裂开始之前停止，并且伴随着DSB的缺失。为了研究这种现象的潜在机制，在野生型菌株和CYC 2敲除菌株之间进行完整的转录组谱。RNA-Seq结果的功能分析确定了包括SPO 11在内的相关差异表达基因（DEG），这些DEG在同源重组（HR）中富含DNA修复/错配修复（MMR）术语，这表明CYC 2可以通过调节SPO 11并参与HR在减数分裂中发挥关键作用。本文的在线版本（doi：10.1007/s13238-016-0254-9）包含补充材料，可供授权用户使用。
Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks (DSBs) made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5–3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes (DEGs) including SPO11 and these DEGs are enriched in DNA repair/mismatch repair (MMR) terms in homologous recombination (HR), which indicates that CYC2 could play a crucial role in meiosis by regulating SPO11 and participating in HR. The online version of this article (doi:10.1007/s13238-016-0254-9) contains supplementary material, which is available to authorized users.