Genome-Wide Identification and Transcriptome-Based Expression Profiling of the Sox Gene Family in the Nile Tilapia (Oreochromis niloticus).
Genome-Wide Identification and Transcriptome-Based Expression Profiling of the Sox Gene Family in the Nile Tilapia (Oreochromis niloticus).
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尼罗罗非鱼 (Oreochromis niloticus) Sox 基因家族的全基因组鉴定和基于转录组的表达谱
DOI：
10.3390/ijms17030270
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发表时间：
2016-02-23
影响因子：
5.6
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Wei L;Yang C;Tao W;Wang D
Sox转录因子家族的特点是存在一个sry相关的高迁移群（high-mobility group， HMG）盒子，在动物的各种生物过程中发挥重要作用，包括性别决定和分化，以及多器官的发育。本研究在尼罗罗非鱼（Oreochromis niloticus）基因组中鉴定出27个Sox基因，并将其分为7个类群。罗非鱼Sox基因的每一组成员都表现出相对保守的外显子-内含子结构。对比分析表明，Sox基因家族在罗非鱼和其他硬骨鱼全基因组复制后发生了扩增，K族仅存在于硬骨鱼中。基于转录组的分析表明，大多数罗非鱼Sox基因在性腺发育过程中呈现阶段特异性和/或性别二态表达，其中6个B组Sox基因在成人大脑中特异性表达。本研究结果有助于进一步了解罗非鱼Sox基因家族的基因结构和时空表达，并有助于进一步解读Sox基因在硬骨鱼性别决定和性腺发育中的作用。
The Sox transcription factor family is characterized with the presence of a Sry-related high-mobility group (HMG) box and plays important roles in various biological processes in animals, including sex determination and differentiation, and the development of multiple organs. In this study, 27 Sox genes were identified in the genome of the Nile tilapia (Oreochromis niloticus), and were classified into seven groups. The members of each group of the tilapia Sox genes exhibited a relatively conserved exon-intron structure. Comparative analysis showed that the Sox gene family has undergone an expansion in tilapia and other teleost fishes following their whole genome duplication, and group K only exists in teleosts. Transcriptome-based analysis demonstrated that most of the tilapia Sox genes presented stage-specific and/or sex-dimorphic expressions during gonadal development, and six of the group B Sox genes were specifically expressed in the adult brain. Our results provide a better understanding of gene structure and spatio-temporal expression of the Sox gene family in tilapia, and will be useful for further deciphering the roles of the Sox genes during sex determination and gonadal development in teleosts.