Abscopal effect of boron neutron capture therapy (BNCT): proof of principle in an experimental model of colon cancer
Abscopal effect of boron neutron capture therapy (BNCT): proof of principle in an experimental model of colon cancer
复制标题
硼中子俘获疗法（BNCT）的远隔效应：结肠癌实验模型的原理证明
DOI：
10.1007/s00411-017-0704-7
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发表时间：
2017
影响因子：
1.7
通讯作者：
A. Schwint
中科院分区：
文献类型：
--
作者：
V. Trivillin;E. Pozzi;L. Colombo;S. Thorp;M. A. Garabalino;A. Monti Hughes;S. González;R. O. Farías;P. Curotto;G. S. Santa Cruz;D. Carando;A. Schwint
本研究的目的是首次评估硼中子俘获疗法（BNCT）的远隔效应。 26 只 BDIX 大鼠右后胁皮下皮下接种 1 × 106 DHD/K12/TRb 同系结肠癌细胞。接种后三周，在位于阿根廷布宜诺斯艾利斯的RA-3核反应堆中，对12只带有肿瘤结节的大鼠的右腿进行BPA-BNCT（BPA-硼苯丙氨酸）治疗，皮肤吸收剂量为7.5 Gy作为剂量限制组织。其余组14只荷瘤大鼠不予治疗并用作对照。 BNCT 后两周，将 1 × 106 DHD/K12/TRb 细胞皮下注射到 26 只 BDIX 大鼠每只的对侧左后胁腹中。连续 7 周每周测量双腿的肿瘤体积，以确定右腿 BNCT 的反应，并评估右腿 BNCT 对左腿肿瘤发展的潜在影响。在 BNCT 组中，右腿肿瘤对 BNCT 有反应的动物（治疗后/治疗前肿瘤体积 <1）与无反应的动物（治疗后/治疗前≥1）相比，观察到对侧左侧肿瘤体积有统计学意义的显着减少，即 13 ± 15 vs 271 ± 128 mm3。此外，与未治疗的动物相比，BNCT 反应性动物（治疗后/治疗前 <1）对侧左腿肿瘤体积显着减少，即 13 ± 15 vs 254 ± 251 mm3。目前在简单动物模型中进行的研究提供了原理证明，即肿瘤对 BNCT 的阳性反应能够诱导远隔效应。
The aim of the present study was to evaluate, for the first time, the abscopal effect of boron neutron capture therapy (BNCT). Twenty-six BDIX rats were inoculated subcutaneously with 1 × 106 DHD/K12/TRb syngeneic colon cancer cells in the right hind flank. Three weeks post-inoculation, the right leg of 12 rats bearing the tumor nodule was treated with BPA-BNCT (BPA-Boronophenylalanine) at the RA-3 nuclear reactor located in Buenos Aires, Argentina, at an absorbed dose of 7.5 Gy to skin as the dose-limiting tissue. The remaining group of 14 tumor-bearing rats were left untreated and used as control. Two weeks post-BNCT, 1 × 106 DHD/K12/TRb cells were injected subcutaneously in the contralateral left hind flank of each of the 26 BDIX rats. Tumor volume in both legs was measured weekly for 7 weeks to determine response to BNCT in the right leg and to assess a potential influence of BNCT in the right leg on tumor development in the left leg. Within the BNCT group, a statistically significant reduction was observed in contralateral left tumor volume in animals whose right leg tumor responded to BNCT (post-treatment/pre-treatment tumor volume <1) versus animals who failed to respond (post/pre ≥1), i.e., 13 ± 15 vs 271 ± 128 mm3. In addition, a statistically significant reduction in contralateral left leg tumor volume was observed in BNCT-responsive animals (post/pre <1) vs untreated animals, i.e., 13 ± 15 vs 254 ± 251 mm3. The present study performed in a simple animal model provides proof of principle that the positive response of a tumor to BNCT is capable of inducing an abscopal effect.
DOI：
10.1016/j.ijrobp.2012.03.017
发表时间：
2013-02-01
影响因子：
7
作者：
Stamell, Emily F.;Wolchok, Jedd D.;Gnjatic, Sacha;Lee, Nancy Y.;Brownell, Isaac
通讯作者：
Brownell, Isaac
影响因子：
11.2
作者：
K. Camphausen;M. Moses;C. Ménard;M. Sproull;W. Beecken;J. Folkman;M. O’Reilly
通讯作者：
K. Camphausen;M. Moses;C. Ménard;M. Sproull;W. Beecken;J. Folkman;M. O’Reilly
与手术切除不同，原发肿瘤的照射增强了远端部位的血管生成抑制：宿主与肿瘤相互作用的潜在作用。
影响因子：
11.2
作者：
Hartford,AC;Gohongi,T;Fukumura,D;Jain,RK
通讯作者：
Jain,RK