Seminal plasma α-glucosidase activity and male infertility
Seminal plasma α-glucosidase activity and male infertility
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DOI：
10.1093/humrep/13.3.591
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发表时间：
1998-03-01
影响因子：
6.1
通讯作者：
Comhaire, FH
中科院分区：
文献类型：
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作者：
Mahmoud, AM;Geslevich, J;Comhaire, FH
α-葡糖苷酶的测量（α-GLUC）活性，通过使用市售试剂盒的简单比色试验测定（EpiScreen（R）; FertiPro，Lotenhulle，Belgium）产生的结果与通过荧光参比方法测量的中性同工酶获得的值强烈相关（r = 0.85，P = 0.003，n = 13），前一种方法的内、间变异系数小（分别为6.6%和4.3%），无精子症输精管切除男性（n = 27）精液中的α-GLUC活性显著低于存在残留精子的输精管切除男性（n = 11，P < 0.01）与原发于睾丸的无精子症男性受试者工作曲线（ROC）分析显示，在标准值13.5 U/l时，α-GLUC测量在区分梗阻性与睾丸性无精子症方面具有合理的准确性（敏感性= 82%，特异性= 70%），在存在精子的情况下，α-GLUC活性和每次射精量与精子浓度呈正相关（r = 0.53和0.38，n = 472），线速度（r = 0.35和0.30，n = 224），曲线速度（r = 0.32和r = 0.29，n = 224），（r = 0.35和0.26，n = 64），5 α-二氢睾酮浓度（r = 0.31和0.29，n = 74）和精浆γ-谷氨酰转移酶活性（r = 0.62和0.32，n = 275）。在12-肉豆蔻酸，13-乙酸佛波酯刺激后，α-GLUC活性与ROS生成呈负相关（r =-0.27，n = 104），α-GLUC活性和总产量均与样本中过氧化物酶阳性白色血细胞浓度呈负相关，Vith大于或等于1x 10（6）/ml的这些细胞（r = -0.30和-0.19，n = 165），它的结论是，简单的光度测量的α-精浆中GLUC活性反映了附睾的功能状态，可能有助于某些无精子症的鉴别诊断。
Measurement of alpha-glucosidase (alpha-GLUC) activity by means of a simple colorimetric test using a commercial kit (EpiScreen(R); FertiPro, Lotenhulle, Belgium) yielded results that were strongly correlated with the values obtained for the neutral iso-enzyme measured by a fluorimetric reference method (r = 0.85, P = 0.003, n = 13), The former method was characterized by a low intra-and inter-coefficient of variation (6.6 and 4.3% respectively), Vasectomized men with azoospermia (n = 27) had a significantly lower alpha-GLUC activity in semen than vasectomized men with residual spermatozoa present (n = 11, P < 0.01) and men with azoospermia of primary testicular origin (n = 33, P < 0.01), Receiver operating curve (ROC) analysis showed alpha-GLUC measurement to be reasonably accurate in differentiation between cases with obstructive versus testicular azoospermia at criterion value 13.5 U/l (sensitivity = 82%, specificity = 70%), In cases with spermatozoa present, alpha-GLUC activity and output per ejaculate were positively correlated with sperm concentration (r = 0.53 and 0.38, n = 472), linear velocity (r = 0.35 and 0.30, n = 224), curvilinear velocity (r = 0.32 and r = 0.29, n = 224), semen adenosine triphosphate (r = 0.35 and 0.26, n = 64), the concentration of 5 alpha-dihydrotestosterone (r = 0.31 and 0.29, n = 74), and gamma-glutamyltransferase activity (r = 0.62 and 0.32, n = 275) in seminal plasma. The activity of alpha-GLUC was inversely correlated with ROS generation after 12-myristate, 13-acetate phorbol ester stimulation (r = -0.27, n = 104), and both alpha-GLUC activity and total output were inversely correlated with the concentration of peroxidase-positive white blood cells among samples,vith greater than or equal to 1x10(6)/ml of these cells (r = -0.30 and -0.19, n = 165), It is concluded that simple photometric measurement of alpha-GLUC activity in seminal plasma reflects the functional state of the epididymis and may be helpful for the differential diagnosis of certain cases with azoospermia.