ANTIBODIES DIRECTED AGAINST ANTIGENS ON THE ENDOTHELIUM OF PERITUBULAR CAPILLARIES IN PATIENTS WITH REJECTING RENAL ALLOGRAFTS
ANTIBODIES DIRECTED AGAINST ANTIGENS ON THE ENDOTHELIUM OF PERITUBULAR CAPILLARIES IN PATIENTS WITH REJECTING RENAL ALLOGRAFTS
复制标题
针对拒绝同种异体肾移植患者的管周毛细血管内皮抗原的抗体
DOI：
10.1097/00007890-197903000-00007
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发表时间：
1979
期刊：
影响因子：
6.2
通讯作者：
J. Graeff
中科院分区：
文献类型：
--
作者：
L. Paul;L. van Es;J. V. van Rood;A. Leeuwen;G. de la Riviere;J. Graeff
本研究旨在检测人类同种异体肾移植受者对供肾内皮抗原的体液免疫反应。采用间接免疫荧光技术对61例接受62例移植物移植的受者血清中循环内皮抗体（CEAb）的存在进行了研究，并将移植物的移植前活检作为底物。移植后7周内出现排斥反应的10例患者中有6例血清中存在针对小管周围毛细血管内皮的IgG抗体，而无排斥反应的患者血清中未发现IgG抗体（P < 0.001）。移植后活检的免疫荧光研究显示IgG仅在CEAb患者的移植物中沿小管周围毛细血管内皮存在。间接免疫荧光技术显示，这些移植物的洗脱液含有与供体内皮结合的IgG抗体。用人血小板或Wistar大鼠红细胞吸收内皮抗体（EAb）阳性血清表明，EAb不针对血清学上确定的HLA抗原或大鼠红细胞上的嗜异性抗原。我们从这项研究中得出结论，针对内皮抗原的抗体的存在与移植物预后不良有关，这些抗体可能是排斥过程的原因。
SUMMARY This study was undertaken to examine the humoral immune response against endothelial antigens of the donor kidney in human renal allograft recipients. Sera from 61 transplant recipients who received 62 grafts were studied for the presence of circulating endothelial antibodies (CEAb) using an indirect immunofluorescence technique with a pretransplant biopsy of the graft as a substrate. IgG antibodies directed against the endothelium of peritubular capillaries were found in the sera of 6 of the 10 patients with graft rejection within 7 weeks after transplantation, whereas these antibodies were not found in the absence of rejection (P < 0.001). Immunofluorescence studies of post-transplant biopsies showed IgG along the endothelium of peritubular capillaries only in the grafts of patients with CEAb. Eluates from these grafts contained IgG antibodies that bound to the endothelium of the donor as shown by the indirect immunofluorescence téchnique. Absorption of endothelial antibody (EAb)-positive sera with human platelets or Wistar strain rat erythrocytes showed that the EAb were not directed against serologically defined HLA antigens or against heterophile antigens on rat erythrocytes. We conclude from this study that the presence of antibodies directed against endothelial antigens is associated with poor graft prognosis and that these antibodies may be responsible for the rejection process.