Visualized single cell dynamics and analysis of molecular tricks
Visualized single cell dynamics and analysis of molecular tricks
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DOI：
10.1016/s0003-2670(99)00704-7
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发表时间：
1999-11-01
影响因子：
6.2
通讯作者：
Masujima, T
中科院分区：
文献类型：
--
作者：
Masujima, T
通过视频显微镜和质谱仪在单细胞水平上分析了各种细胞的动态行为。通过对单个细胞的视频图像分析，可以可视化细胞行为的真实的动态时刻，这些动态时刻以前只能通过想象来获得，例如微粒的爆裂和嗜中性粒细胞的胞吐;肥大细胞模型细胞系RBL-2 H3细胞和胰腺β细胞模型细胞系MIN-6。结合对行为的观察，更直接的分子分析对于显示细胞中的分子相互作用变得更加重要。将质谱的组合应用于单细胞水平的分析。基于这些分析的当前进展，预测了单细胞动力学分析的未来趋势。(C)1999 Elsevier Science B. V.保留所有权利。
The dynamic behaviours of various cells were analysed by video-microscopes and mass spectrometers at the single-cell level. By the video image analysis of a single cell, real dynamic moments of cell behaviour, which were previously accessible only to the imagination, can be visualized - such as the popping of micro-granules, and the exocytosis of neutrophils; a mast cell model cell line, RBL-2H3 cells, and pancreatic beta cell model cell line, MIN-6. In combination with the observations of behaviour, more direct molecular analyses become much more important for showing the molecular interplay in a cell. The combination of mass spectrometries was applied to analysis at the single cellular level. Based on the current progress in these analyses, the future trends in analyses of single cellular dynamism are predicted. (C) 1999 Elsevier Science B.V. All rights reserved.