MicroRNA-4331-5p promotes FMDV replication through inhibiting interferon pathways in PK-15 cells
MicroRNA-4331-5p promotes FMDV replication through inhibiting interferon pathways in PK-15 cells
复制标题
MicroRNA-4331-5p 通过抑制 PK-15 细胞中的干扰素途径促进 FMDV 复制
DOI：
10.1016/j.virusres.2020.198064
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发表时间：
2020-09-01
期刊：
影响因子：
5
通讯作者：
Sun, Yuefeng
中科院分区：
文献类型：
--
作者：
Ren, Tingting;Wang, Yanxue;Sun, Yuefeng
在病毒侵袭过程中，microrna在调节病原体和宿主细胞之间的战斗中起着至关重要的作用。MiR-4331加重传染性胃肠炎病毒（TGEV）诱导的线粒体损伤，也通过靶向细胞CDCA7抑制TGEV基因7的转录。否则，miR-4331-5p通过靶向病毒HA和NS影响H1N1/2009甲型流感病毒的复制。然而，microRNA ssc-miR-4331-5p （miR-4331-5p）是否调控口蹄疫病毒（FMDV）的复制尚不清楚。为了探讨miR-4331-5p在FMDV感染中的作用，我们检测了miR-4331-5p在猪肾（PK-15）细胞中的表达水平。结果表明，FMDV感染可直接上调miR-4331-5p的表达，而转染miR-4331-5p的模拟物或抑制剂可促进或抑制FMDV的复制。进一步的研究清楚地表明，miR-4331-5p通过抑制I型干扰素途径增加FMDV复制。这些数据表明miR-4331-5p在调控FMDV复制中发挥重要作用。
MicroRNAs play vital roles in regulating the battle between pathogens and host cells during viral challenging. MiR-4331 aggravates transmissible gastroenteritis virus (TGEV) -induced mitochondrial damage, also suppresses transcription of TGEV gene 7 via targeting cellular CDCA7. Otherwise, miR-4331-5p affects H1N1/2009 influenza A virus replication by targeting viral HA and NS. However, whether microRNA ssc-miR-4331-5p (miR-4331-5p) regulates foot and mouth virus (FMDV) replication remains unclear. To explore the role of miR-4331-5p in FMDV infection, we detected the expression level of miR-4331-5p in porcine kidney (PK-15) cells. The results showed that FMDV infection directly upregulates miR-4331-5p expression, while transfection of mimics or inhibitor of miR-4331-5p promotes or inhibits FMDV replication. Further investigation clearly showed that miR-4331-5p increases FMDV replication through inhibiting type I interferon pathways. These data demonstrate that miR-4331-5p plays an important role in regulating FMDV replication.