Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster.
Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster.
复制标题
日本火腹蝾螈（Cynopspyrrhogaster）的 15 个微卫星标记的表征。
DOI：
10.1007/s12686-013-0104-5
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Inoue-Murayama M
中科院分区：
文献类型：
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作者：
Tominaga A;Matsui M;Yoshikawa N;Hayano A;Inoue-Murayama M
日本火腹蝾螈（Cynops pyrrhogaster）是日本大陆特有的一种，在日本的红色名录中被列为近危。然而，有效的微卫星标记，估计其遗传多样性和结构的保护措施还没有报道。因此，我们开发了15个新的微卫星标记，该物种使用表达序列标签和下一代测序数据。每个位点的等位基因数和期望杂合度分别为3-28（平均值= 11.6）和0-0.97。这些标记将为研究该属植物的遗传多样性和遗传结构以及保护管理提供依据。红腹菌
The Japanese fire-bellied newtCynops pyrrhogaster, endemic to mainland Japan is ranked as Near Threatened in the red list of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed fifteen novel microsatellite markers for this species using expressed sequence tag and next-generation sequencing data. The number of alleles and expected heterozygosity at each locus were 3–28 (mean = 11.6) and 0–0.97. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management ofC. pyrrhogaster.