One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein
One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein
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DOI：
10.1128/jb.184.16.4617-4619.2002
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发表时间：
2002-08-01
影响因子：
3.2
通讯作者：
Pouwels, PH
中科院分区：
文献类型：
--
作者：
Smit, E;Pouwels, PH
当与绿色荧光蛋白融合时，嗜酸乳杆菌 S 蛋白的 N 端重复序列 (SAC1) 可以有效且特异性地与细胞壁片段 (CWF) 结合，而 C 端重复序列 (SAC2) 则不能。用氢氟酸而非苯酚处理 CWF 会阻止结合。显然，SAC1 对于细胞壁结合是必要且充分的。我们的数据表明，SAC 将 S 蛋白锚定在细胞壁磷壁酸上。
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding, Apparently, SAC1 is necessary and sufficient for cell wall binding. Our data suggest that SAC anchors the S-protein to a cell wall teichoic acid.