Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans
Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans
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DOI：
10.1093/glycob/cwl044
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发表时间：
2006-12-01
期刊：
影响因子：
4.3
通讯作者：
Blixt, Ola
中科院分区：
文献类型：
--
作者：
Bohorov, Ognian;Andersson-Sand, Hillevi;Blixt, Ola
聚糖阵列开发受到有效生成具有伯胺或其他官能团的游离还原聚糖的衍生物的复杂性的限制。合成了一种新型的双官能间隔基，其对游离聚糖具有选择性反应性，并具有第二官能度，即伯胺。我们证明了一种有效的一步衍生化的各种聚糖，包括天然分离的N-聚糖，O-聚糖，牛奶寡糖，和细菌多糖在微克规模。不需要保护基操作或异头中心的活化。为了证明其在聚糖微阵列制造中的实用性，我们比较了具有不同胺间隔物的聚糖在胺反应性玻璃表面上的掺入。我们的研究结果表明，与这种双功能接头缀合的聚糖被有效地打印并被各种凝集素和抗体检测。
Glycan array development is limited by the complexity of efficiently generating derivatives of free reducing glycans with primary amines or other functional groups. A novel bi-functional spacer with selective reactivity toward the free glycan and a second functionality, a primary amine, was synthesized. We demonstrated an efficient one-step derivatization of various glycans including naturally isolated N-glycans, O-glycans, milk oligosaccharides, and bacterial polysaccharides in microgram scale. No protecting group manipulations or activation of the anomeric center was required. To demonstrate its utility for glycan microarray fabrication, we compared glycans with different amine-spacers for incorporation onto an amine-reactive glass surface. Our study results revealed that glycans conjugated with this bi-functional linker were effectively printed and detected with various lectins and antibodies.