Harzianone Biosynthesis by the Biocontrol Fungus Trichoderma
Harzianone Biosynthesis by the Biocontrol Fungus Trichoderma
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DOI：
10.1002/cbic.201700462
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发表时间：
2017-12-05
期刊：
影响因子：
3.2
通讯作者：
Dickschat, Jeroen S.
中科院分区：
文献类型：
--
作者：
Barra, Lena;Dickschat, Jeroen S.
通过使用闭环汽提装置（CLSA）分析由木霉属的七种真菌菌株产生的挥发性萜烯，揭示了共同产生哈茨酮（harzianone），哈茨酮是由稠合四环4，7，5，6-元环系统组成的生物活性的、结构独特的二萜类化合物。萜烯环化机理通过使用选择性C-13-和H-2-标记的合成甲羟戊酸内酯同位素体的喂养实验进行研究，随后通过（CNMR）-C-13光谱和GC/MS分析掺入模式。哈茨酮的结构进一步得到体内产生的完全C-13-标记的二萜的C-13，（CCOSY）-C-13实验的支持。
Analysis of the volatile terpenes produced by seven fungal strains of the genus Trichoderma by use of a closed-loop stripping apparatus (CLSA) revealed a common production of harzianone, a bioactive, structurally unique diterpenoid consisting of a fused tetracyclic 4,7,5,6-membered ring system. The terpene cyclization mechanism was studied by feeding experiments using selectively C-13- and H-2-labeled synthetic mevalonolactone isotopologues, followed by analysis of the incorporation patterns by (CNMR)-C-13 spectroscopy and GC/MS. The structure of harzianone was further supported from a C-13,(CCOSY)-C-13 experiment of the in-vivo-generated fully C-13-labeled diterpene.