THIOL‐GLYOXYLATE ADDUCTS AS SUBSTRATES FOR RAT KIDNEY L‐α‐HYDROXY ACID OXIDASE *
THIOL‐GLYOXYLATE ADDUCTS AS SUBSTRATES FOR RAT KIDNEY L‐α‐HYDROXY ACID OXIDASE *
复制标题
硫醇-乙醛酸加合物作为大鼠肾 L-α-羟基酸氧化酶的底物 *
DOI：
10.1111/j.1749-6632.1982.tb21441.x
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发表时间：
1982
影响因子：
5.2
通讯作者：
G. A. Hamilton
中科院分区：
文献类型：
--
作者：
E. Brush;G. A. Hamilton
过氧化物体是一种含有过氧化氢酶和几种氧化酶的小细胞器，包括黄素蛋白氧化酶、L-α-羟基酸氧化酶(L)和D-氨基酸氧化酶(D-AAO)。‘--虽然这些细胞器几乎存在于所有动物细胞中，但它们的代谢功能尚不清楚。黄素蛋白氧化酶的具体功能仍然不清楚，因为直到最近，关于这些酶的生理底物还没有提出合理的建议，而且已知的良好底物在生理上没有任何显著的程度。最近我们报道了乙醛和各种胺的加合物是D-AAO的底物，我们提出了这种酶最有可能的生理底物是半胱胺-乙醛加合物，噻唑烷-2-羧酸盐。因为任何亲核剂与乙醛的加合物都是a-羟基酸(等式)。1)。X=0，S，NH RXH+0=CH-COORX-CH-COO(1)IOH
Peroxisomes are small organelles that characteristically contain catalase and several oxidative enzymes, including the flavoprotein oxidases, L-a-hydroxy acid oxidase (L-HAO) and D-aminO acid oxidase (D-AAO).'-~ Although these organelles are present in virtually all animal cells,' their metabolic function has remained unknown. The specific function of the flavoprotein oxidases has remained even more obscure because, until recently, no reasonable suggestion concerning the physiological substrates for these enzymes has been made, and the known, good substrates are not present to any significant extent physiologically. Recently we reported' that adducts of glyoxylate and various amines are substrates for D-AAO, and we presented arguments that the most likely physiological substrate for this enzyme is the cysteamine-glyoxylate adduct, thiazolidine-2-carboxylate. Because an adduct of any nucleophile with glyoxylate would be an a-hydroxy acid (eq. 1). X = 0, S, NH RXH + 0 = CH-COORX-CH-COO(1) I OH