Quantitative shotgun proteomics of enriched heterocysts from Nostoc sp.: PCC 7120 using 8-plex isobaric peptide tags
Quantitative shotgun proteomics of enriched heterocysts from Nostoc sp.: PCC 7120 using 8-plex isobaric peptide tags
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DOI：
10.1021/pr700604v
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发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Wright, Phillip C.
中科院分区：
文献类型：
--
作者：
Ow, Saw Yen;Cardona, Tanai;Wright, Phillip C.
丝状蓝细菌念珠藻属菌株PCC 7120能够固定大气氮。核心过程的不稳定性质需要营养细胞的终末分化以形成异形胞，异形胞是具有改变的细胞和代谢基础结构的特化细胞以介导N-2固定过程。我们提出了一项调查，针对细胞蛋白质组表达的异形胞相比，营养细胞的N-2固定条件下培养的人口。在富集的重复异型胞和营养细胞上使用新的8重iTRAQ试剂，并重复N-2固定和非N-2固定细丝以实现准确测量。通过这种方法，我们成功地鉴定了506种蛋白质，其中402种蛋白质具有可靠的定量。通过纯化的异形胞分析提供的观察结果使得能够阐明各自细胞类型之间的主导代谢过程，而对细丝的强调使得能够进行总体比较。本调查提供的分析水平提出了各种工具和知识，对蓝藻生物制氢的未来发展是重要的。
The filamentous cyanobacterium Nostoc sp. strain PCC 7120 is capable of fixing atmospheric nitrogen. The labile nature of the core process requires the terminal differentiation of vegetative cells to form heterocysts, specialized cells with altered cellular and metabolic infrastructure to mediate the N-2-fixing process. We present an investigation targeting the cellular proteomic expression of the heterocysts compared to vegetative cells of a population cultured under N-2-fixing conditions. New 8-plex iTRAQ reagents were used on enriched replicate heterocyst and vegetative cells, and replicate N-2-fixing and non-N-2-fixing filaments to achieve accurate measurements. With this approach, we successfully identified 506 proteins, where 402 had confident quantifications. Observations provided by purified heterocyst analysis enabled the elucidation of the dominant metabolic processes between the respective cell types, while emphasis on the filaments enabled an overall comparison. The level of analysis provided by this investigation presents various tools and knowledge that are important for future development of cyanobacterial biohydrogen production.