A SIMPLE METHOD FOR MAINTAINING LARGE, AGING POPULATIONS OF CAENORHABDITIS-ELEGANS
A SIMPLE METHOD FOR MAINTAINING LARGE, AGING POPULATIONS OF CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0047-6374(80)90090-1
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发表时间：
1980-01-01
影响因子：
5.3
通讯作者：
RAOSANADI, D
中科院分区：
文献类型：
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作者：
GANDHI, S;SANTELLI, J;RAOSANADI, D
一种能够建立和维持大的、年龄同步的线虫C。elegans描述。该技术有3个基本组成部分：富含化学物质的培养基;生产和收获大量卵子的方法;和5-氟脱氧尿苷（FUdR），一种DNA合成抑制剂。蠕虫培养物通过玻璃棉或金属丝网过滤以分离幼幼虫。这些蠕虫在成熟后产卵，收集4-6小时并孵化。在幼虫即将成熟之前加入低水平的FUdR（25 μ M）。这个时机对于避免发育异常很重要。成虫在FUdR存在下产卵，但卵不孵化，这保持了培养的同步性。许多老化特征似乎是相似的治疗和未治疗的蠕虫，如停止产卵的时间，可见的和行为的年龄相关的变化的外观，和平均寿命。该系统似乎适合于大规模的生化分析的某些方面的老化在C。优美的
A technique capable of establishing and maintaining large, age-synchronous populations of the nematode C. elegans is described. The technique has 3 essential components: a rich chemical medium; a method for producing and harvesting mass quantities of eggs; and 5-fluorodeoxyuridine (FUdR), an inhibitor of DNA synthesis. A culture of worms is filtered through glass wool or a wire screen to isolate young larvae. Eggs laid by these worms after they mature are collected over a period of 4-6 h and allowed to hatch. A low level of FUdR (25 .mu.M) is added just before the larvae reach maturity. This timing is important in avoiding developmental abnormalities. The adults lay eggs in the presence of FUdR but the eggs do not hatch, which maintains the synchrony of the culture. Many aging characteristics appear to be similar in treated and untreated worms, such as the time of cessation of egg production, the appearance of visible and behavioral age-related changes, and the mean lifespan. This system seems suitable for large-scale biochemical analysis of certain aspects of aging in C. elegans.