Emulsifying Properties of an Homologous Series of Medium- and Long-Chain D-Maltotriose Esters and their Impacts on the Viabilities of Selected Cell Lines
Emulsifying Properties of an Homologous Series of Medium- and Long-Chain D-Maltotriose Esters and their Impacts on the Viabilities of Selected Cell Lines
复制标题
中链和长链 D-麦芽三糖同源系列的乳化特性及其对选定细胞系活力的影响
DOI：
10.1021/acs.jafc.0c02890
复制
发表时间：
2020
影响因子：
6.1
通讯作者：
Lan Ping
中科院分区：
文献类型：
--
作者：
Zhu Jian-Peng;Ma Ya-Ru;Teng Yinglai;Chen Jing;Banwell Martin G.;Lan Ping
用于食品工业的功能性和营养性表面活性剂的开发仍然引起人们极大的兴趣。在本研究中，通过酶法制备了一系列带有10-18个碳烷基侧链的6″-O-酰基麦芽三糖单酯。由那些掺有棕榈酰基、硬脂酰基和油酰基侧链的单酯衍生的乳液通常表现出有利的保质期、对环境变化的优异抵抗力、更有利的液滴尺寸分布以及针对各种癌细胞系的更强的细胞毒性作用。 Ester6 通过诱导 G1 期阻滞显着抑制 MCF-7 乳腺癌细胞的增殖。具体来说，这种特殊的酯抑制了 G1 期相关标记物细胞周期蛋白 D1 和细胞周期蛋白 E 以及周期依赖性激酶 4 的水平。因此，这项研究表明，麦芽三糖酯不仅可以用作新型功能性食品乳化剂，而且在体外通过明确的作用机制发挥显着的细胞毒性剂的作用。
The development of functional as well as nutritional surfactants for the food industry remains a matter of great interest. In the present study, a series of 6″-O-acylmaltotriose monoesters bearing alkyl side chains of 10–18 carbons was prepared by enzymatic means. The emulsions derived from those monoesters incorporating palmitoyl, stearoyl, and oleoyl side chains generally displayed advantageous shelf-lives, superior resistance to environmental variations, and more favorable droplet size distributions as well as stronger cytotoxic effects against various cancer cell lines. Ester6was shown to significantly inhibit the proliferation of MCF-7 breast cancer cells by inducing G1 phase arrest. Specifically, the levels of the G1 phase-related markers cyclin D1 and cyclin E as well as the cycle-dependent kinase 4 were suppressed by this particular ester. This study thus reveals that maltotriose esters can not only serve as novel functional food emulsifiers but also act,in vitro, as notable cytotoxic agents through a well-defined mechanism-of-action.