Overexpression of the Arabidopsis DREB1A gene enhances potato drought-resistance
Overexpression of the Arabidopsis DREB1A gene enhances potato drought-resistance
复制标题
拟南芥DREB1A基因的过度表达增强马铃薯的抗旱性
DOI：
10.1134/s1021443716040063
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发表时间：
2016-07-01
影响因子：
1.4
通讯作者：
Wang, W. T.
中科院分区：
文献类型：
--
作者：
Jia, X. X.;Li, Y. T.;Wang, W. T.
干旱是限制马铃薯（Solanum tuberosum L.）产量的主要环境胁迫。转录因子DREB1A/CBF3与拟南芥脱水响应元件（DRE/CRT）特异性相互作用，诱导环境胁迫耐受相关基因的表达。本研究通过农杆菌介导的转化，将拟南芥DREB1A基因在马铃薯品种龙树3号（L3）中过表达。采用PCR、Southern blotting和半定量RT-PCR分析评估DREB1A的转化和过表达。结果清楚地证实了DREB1A基因成功整合到基因组中并表达。15 ~ 16叶盆栽植物在干旱胁迫下断水8 d后，非转基因和转基因L3植株叶片超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性、MDA含量和电解质渗漏均增加。转基因植物叶片中SOD、CAT和POD的平均活性分别比非转基因L3提高69.77、60.78和24.60%。非转基因L3植株叶片的丙二醛含量和电解质泄漏量分别比转基因植株叶片增加59.09和37.63%。当不浇水14 d时，对照植株表现出严重的萎蔫，转基因植株仅部分萎蔫。这些结果表明，DREB1A的过表达导致了仙丹植物对干旱胁迫的耐受性提高。
Drought is a major environmental stress that limits potato (Solanum tuberosum L.) production worldwide. The transcription factor DREB1A/CBF3 specifically interacts with the dehydration responsive element (DRE/CRT) and induces expression of genes involved in environmental stress tolerance in Arabidopsis thaliana. In this study, DREB1A of A. thaliana was overexpressed in a potato cultivar Longshu 3 (L3) through Agrobacterium tumefaciens-mediated transformation. The transformation and overexpression of DREB1A were assessed using PCR, Southern blotting and semi-quantitative RT-PCR analysis. The results clearly confirmed that the DREB1A gene was successfully integrated into the genome and expressed. When pot-grown plants with 15-16 leaves were subjected to drought stress treatments by withholding water for 8 days, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), the MDA content and electrolyte leakage in leaves from both non-transgenic and transgenic L3 plants leaves increased. The average activities of SOD, CAT, and POD in transgenic plant leaves, respectively showed 69.77, 60.78, and 24.60% increase than those of non-transgenic L3. The MDA content and electrolyte leakage in non-transgenic L3 plant leaves, respectively increased 59.09 and 37.63% relative to those of transgenic plant leaves. When water was withheld for 14 days control plants exhibited severe wilting and transgenic plants only partially wilting. These results demonstrated that overexpression of DREB1A resulted in improved drought stress tolerance in S. tuberosum plants.