Distribution of AAV2-hAADC-transduced cells after 3 years in Parkinsonian monkeys
Distribution of AAV2-hAADC-transduced cells after 3 years in Parkinsonian monkeys
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DOI：
10.1097/01.wnr.0000198952.38563.05
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发表时间：
2006-02-06
期刊：
影响因子：
1.7
通讯作者：
Bankiewicz, KS
中科院分区：
文献类型：
--
作者：
Daadi, MM;Pivirotto, P;Bankiewicz, KS
The present report describes for the first time, the stability of recombinant adeno-associated virus serotype 2 (AAV2) human aromatic L-amino acid decarboxylase (hAADC) gene transfer after 3-year survival time in a non-human primate model of Parkinson's disease. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned monkeys were treated with six injections of 30 mu l/site of AAV2-hAADC at a concentration of 2 x 10(12)vg/ml into the caudate and putamen. Stereological analysis revealed a 46.6% increase in the total number of AAV2-hAADC-transduced cells in the striatum between 8 weeks and 3 years after gene transfer survival time. In the 8-week animals, the distribution of the AADC + cells was dispersed and heterogeneous, whereas in the 3-year animals it was widespread and homogenous. Confocal analysis demonstrated that approximately 85% of the AADC + cells were neuronal nuclei immunoreactive.
