Telomerase activity in germline and embryonic cells of Xenopus.
Telomerase activity in germline and embryonic cells of Xenopus.
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DOI：
10.1002/j.1460-2075.1994.tb06620.x
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发表时间：
1994-07
期刊：
影响因子：
--
通讯作者：
L. L. Mantell-L.;C. Greider
中科院分区：
文献类型：
--
作者：
L. L. Mantell-L.;C. Greider
端粒酶是在染色体末端合成端粒重复序列的核糖核蛋白。端粒酶活性参与端粒长度的维持。我们以非洲爪蟾（Xenopus laevis）为模型系统，研究了端粒酶在种系细胞和早期发育过程中的表达。我们在手工解剖的爪蟾VI期卵母细胞细胞核中发现了非进程端粒酶活性。端粒酶活性检测贯穿卵发生和胚胎发生。端粒酶在S期和M期细胞周期提取物中均有活性，表明端粒酶活性不受染色体DNA复制的调节。
Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a non‐processive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.