MUTAGENESIS AND MAPPING OF A MOUSE GENE CLOCK, ESSENTIAL FOR CIRCADIAN BEHAVIOR
MUTAGENESIS AND MAPPING OF A MOUSE GENE CLOCK, ESSENTIAL FOR CIRCADIAN BEHAVIOR
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DOI：
10.1126/science.8171325
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发表时间：
1994-04-29
期刊：
影响因子：
56.9
通讯作者：
TAKAHASHI, JS
中科院分区：
文献类型：
--
作者：
VITATERNA, MH;KING, DP;TAKAHASHI, JS
为了寻找调节哺乳动物昼夜节律的基因，对用N-乙基-N-亚硝基脲（ENU）处理的小鼠的后代进行了昼夜节律钟突变的筛选。一个半显性突变，时钟，延长昼夜节律周期和废除持久性的节奏被确定。Clock作为一个单独的基因被分离出来，定位在小鼠5号染色体的中部，这是一个与人类4号染色体同线的区域。ENU诱变的能力与通过图谱位置克隆小鼠基因的能力相结合，为研究哺乳动物的复杂行为提供了一种普遍适用的方法。
In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.