LOCALIZATION OF RIBOSOMAL-PROTEINS L7L12 IN 50 S SUBUNIT OF ESCHERICHIA-COLI RIBOSOMES BY ELECTRON-MICROSCOPY
LOCALIZATION OF RIBOSOMAL-PROTEINS L7L12 IN 50 S SUBUNIT OF ESCHERICHIA-COLI RIBOSOMES BY ELECTRON-MICROSCOPY
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DOI：
10.1016/s0022-2836(76)80079-4
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
ROTH, HE
中科院分区：
文献类型：
--
作者：
BOUBLIK, M;HELLMANN, W;ROTH, HE
通过电子显微镜（EM）确定了核糖体蛋白L7L12在大肠杆菌核糖体大亚基（50S）上的定位。两种独立方法的结果，即使用针对L7L12的特异性抗体标记以及去除50S亚基中的L7L12蛋白，是一致的，并且表明这些蛋白位于50S核糖体亚基的冠状区域。已确定的冠状结构的不对称性有助于核糖体组分的拓扑定位。L7L12蛋白参与与核糖体上氨酰基和肽酰基位点相关的几种功能；去除和重组实验为将核糖体精细结构的变化与其生物学功能相关联提供了直接的可能性。
The localization of ribosomal proteins L7L12 on the large (50S) subunit of E. coli ribosomes was determined by EM. The results from 2 independent methods, labeling with antibody specific against L7L12 and depletion of the 50S subunits of the proteins L7L12, are in agreement and show that these proteins are located in the crown-shaped region of the 50S ribosomal subunit. The established asymmetry of the crown facilitates the topographical localization of ribosomal constituents. Proteins L7L12 are involved in several functions related to aminoacyl and peptidyl sites on the ribosome; the depletion and reconstitution experiments provide a direct possibility of correlating the changes in the fine structure of ribosomes with their biological function.