Studies of DNA Damage Inhibition by Dietary Antioxidants Using Metallopolyion/DNA Sensors
Studies of DNA Damage Inhibition by Dietary Antioxidants Using Metallopolyion/DNA Sensors
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DOI：
10.1002/elan.200503414
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发表时间：
2006-02
期刊：
影响因子：
3
通讯作者：
Amos Mugweru;J. Rusling
中科院分区：
文献类型：
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作者：
Amos Mugweru;J. Rusling
对含有锇和钌金属聚合物薄膜的伏安传感器进行了评估，以监测抗氧化剂对DNA损伤反应的影响。在第一个例子中，芹菜素、白杨素和抗坏血酸被证明可抑制由芬顿试剂产生的羟基自由基对DNA的氧化。第二个例子涉及含有肌红蛋白（Mb）作为酶模拟物的DNA薄膜，用于将苯乙烯转化为环氧苯乙烷。在此，传感器的钌峰作为核碱基与环氧苯乙烷加合物形成导致DNA损伤的标志。抗氧化剂对环氧苯乙烷本身与DNA薄膜的反应没有影响，但当传感器薄膜中的Mb产生环氧苯乙烷时，微摩尔量的抗氧化剂提供了显著的损伤保护。这表明保护作用源于抗氧化剂在酶转化水平的作用，可能是通过还原蛋白质的活性铁氧基中间体。结果证明了金属聚离子/DNA薄膜传感器在研究DNA损伤抑制方面的有效性。
Voltammetric sensors featuring thin-films containing osmium and ruthenium metallopolymers were evaluated to monitor the influence of antioxidants on DNA damage reactions. In the first example, apigenin, chrysin, and ascorbic acid were shown to inhibit oxidation of DNA by hydroxyl radicals generated with Fenton's reagent. A second example involved films of DNA with myoglobin (Mb) as an enzyme mimic which was used to convert styrene to styrene oxide. Here, the Ru peak of the sensor served as a marker of DNA damage from adduct formation between nucleobases and styrene oxide. There was no influence of the antioxidants on the reaction of styrene oxide itself with DNA films, but significant damage protection was afforded by micromolar amounts of antioxidants when styrene oxide was generated by Mb in the sensor films. This suggests that protection ensued from action of the antioxidants at the enzyme conversion level, probably by reduction of the active ferryloxy intermediate of the protein. Results demonstrate the usefulness of the thin metallopolyion/DNA film sensors in investigations of DNA damage inhibition.