S-nitrosylation triggers ABI5 degradation to promote seed germination and seedling growth.
S-nitrosylation triggers ABI5 degradation to promote seed germination and seedling growth.
复制标题
S-亚硝基化触发 ABI5 降解，促进种子发芽和幼苗生长。
DOI：
10.1038/ncomms9669
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发表时间：
2015-10-23
影响因子：
16.6
通讯作者：
Lorenzo O
中科院分区：
文献类型：
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作者：
Albertos P;Romero-Puertas MC;Tatematsu K;Mateos I;Sánchez-Vicente I;Nambara E;Lorenzo O
植物的存活取决于种子的萌发和通过萌发后发育检查点的进展。这些过程由胁迫植物激素脱落酸（阿坝）控制。阿坝调节碱性亮氨酸拉链转录因子ABI 5，生长抑制的中心枢纽，而活性氮分子一氧化氮（NO）在种子萌发过程中抵消阿坝。然而，种子感觉到更有利的条件并开始发芽的分子机制仍然难以捉摸。在这里，我们表明，ABI 5通过NO促进生长，ABI 5的积累在遗传背景中改变与受损的NO稳态。ABI 5在半胱氨酸-153处的S-亚硝基化促进其通过基于CULLIN 4和KEEP ON GOING E3连接酶的降解，并促进种子萌发。相反，ABI 5在半胱氨酸-153处的突变使蛋白质稳定性和通过NO耗尽对种子萌发的抑制解除调节。这些研究结果表明，NO和阿坝激素信号通过不同的翻译后修饰ABI 5在早期幼苗发育过程中的反向分子联系。 在种子萌发和幼苗生长过程中，一氧化氮抵消植物激素阿坝的抑制作用。在这里，Albertos等人表明，一氧化氮可以通过诱导S-亚硝基化和参与阿坝信号传导的ABI 5转录因子的降解来拮抗阿坝。
Plant survival depends on seed germination and progression through post-germinative developmental checkpoints. These processes are controlled by the stress phytohormone abscisic acid (ABA). ABA regulates the basic leucine zipper transcriptional factor ABI5, a central hub of growth repression, while the reactive nitrogen molecule nitric oxide (NO) counteracts ABA during seed germination. However, the molecular mechanisms by which seeds sense more favourable conditions and start germinating have remained elusive. Here we show that ABI5 promotes growth via NO, and that ABI5 accumulation is altered in genetic backgrounds with impaired NO homeostasis. S-nitrosylation of ABI5 at cysteine-153 facilitates its degradation through CULLIN4-based and KEEP ON GOING E3 ligases, and promotes seed germination. Conversely, mutation of ABI5 at cysteine-153 deregulates protein stability and inhibition of seed germination by NO depletion. These findings suggest an inverse molecular link between NO and ABA hormone signalling through distinct posttranslational modifications of ABI5 during early seedling development. Nitric oxide counteracts the inhibitory effects of the plant hormone ABA during seed germination and seedling growth. Here, Albertos et al. show that nitric oxide can act antagonistically to ABA by inducing the S-nitrosylation and degradation of the ABI5 transcription factor involved in ABA signalling.