Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR
Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR
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DOI：
10.1111/j.1572-0241.2005.40312.x
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发表时间：
2005-02-01
影响因子：
9.8
通讯作者：
Palva, A
中科院分区：
文献类型：
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作者：
Malinen, E;Rinttilä, T;Palva, A
目的：肠道菌群可能与肠易激综合征（IBS）的发生和维持有关。在这项研究中，肠道易激综合征患者的微生物群进行了比较，与对照组没有胃肠道（GI）symptoms.METHODS：粪便微生物群的患者（n = 27）满足罗马II标准的肠易激综合征，进行了比较，与年龄和性别匹配的对照组（n = 22）。每隔3个月采集一次粪便样本。总细菌DNA进行了分析，20定量实时PCR检测，涵盖约300细菌species.RESULTS：广泛的个体差异，观察到肠易激综合征和对照组之间的胃肠道菌群。根据症状亚型（腹泻、便秘和交替优势型）对IBS患者进行分类显示，较低量的乳杆菌属（Lactobacillus spp.）在腹泻型IBS患者的样本中存在Veillonella，而在便秘型IBS患者的样本中，患者携带的Veillonella数量增加。从三个粪便样本的平均结果表明，在肠易激综合征患者（n = 21）和对照组（n = 15）之间的球状梭菌;亚群和链状双歧杆菌组的差异。早期与IBS相关的肠道病原体。无螺杆菌迹象或艰难梭菌被发现，而空肠弯曲菌的一个案例被确定为sequencing.CONCLUSIONS：与这些实时PCR检测，定量改变肠道菌群的IBS patients. Increasing微生物DNA序列的信息将进一步允许设计新的实时PCR检测更广泛的分析肠道微生物在IBS。
OBJECTIVE: The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms.METHODS: Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS, was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species.RESULTS: Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients wheras constipation predominant IBS, patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides; subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS. no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing.CONCLUSIONS: With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS.