Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking
Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking
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DOI：
10.1038/nnano.2016.239
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发表时间：
2017-03-01
影响因子：
38.3
通讯作者：
Kelley, Shana O.
中科院分区：
文献类型：
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作者：
Poudineh, Mahla;Aldridge, PeterM.;Kelley, Shana O.
分析全血中罕见循环肿瘤细胞（ctc）的异质性表型对于揭示这些潜在临床标志物的复杂和动态特性至关重要。这项任务具有挑战性，因为这些细胞在正常血细胞中以十亿分之一的水平存在。在这里，我们报告了一种新的纳米粒子驱动的CTC表征方法，称为磁性排列细胞术，它根据CTC的表面表达表型来描述CTC。我们使用一种微流控芯片来实现这一目标，该芯片成功地处理了全血样本。该方法根据其表型表面标记物的表达对ctc进行单细胞分辨率分类，这些标记物是用磁性纳米颗粒读出的。我们利用这项新技术来揭示小鼠未加工血液中ctc的动态表型，作为肿瘤生长和侵袭性的功能。我们还使用从癌症患者身上收集的血液样本来测试磁排列细胞术。
Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples. The approach classifies CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers, which is read out using magnetic nanoparticles. We deploy this new technique to reveal the dynamic phenotypes of CTCs in unprocessed blood from mice as a function of tumour growth and aggressiveness. We also test magnetic ranking cytometry using blood samples collected from cancer patients.