INDUCTION OF ACROSOME REACTIONS BY THE HUMAN ZONA PELLUCIDA
INDUCTION OF ACROSOME REACTIONS BY THE HUMAN ZONA PELLUCIDA
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DOI：
10.1095/biolreprod38.1.235
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发表时间：
1988-02-01
影响因子：
3.6
通讯作者：
HANSON, FW
中科院分区：
文献类型：
--
作者：
CROSS, NL;MORALES, P;HANSON, FW
我们用两种方法来测试人类透明带诱导人类精子顶体反应的能力。首先，将无活力的人卵母细胞在获能精子（其中少于5%是顶体反应的）的悬浮液中孵育1分钟，以允许每个卵母细胞结合约200个精子。一些卵母细胞立即固定，其余的在没有自由游动精子的情况下再孵育1小时后固定。通过光学显微镜测定，精子上的精子仅为3 . ±. 2%（平均值）.+-. SD），顶体反应在1分钟，并且发生率增加至46 . ±.在接下来的一个小时里，15%。电子显微镜证实，大多数精子在精子顶体上在1分钟是完整的。少数精子处于顶体反应的早期。在随后的一个小时内，在睾丸上发生的顶体反应似乎是形态正常的。第二，用酸分解的透明带（2 - 4个透明带/μ l）处理悬浮液中的精子，使顶体反应精子的发生率从3 . ±. 1%至24 .+-。百分之四我们的结论是，人类透明质酸，或与它密切相关的材料，可以诱导人类精子顶体反应。
We have used two approaches to test the ability of the human zone pellucida to induce acrosome reactions in human sperm. First, nonviable human oocytes were incubated for 1 min in a suspension of capacitated sperm (of which fewer than 5% were acrosome-reacted) to allow binding of about 200 sperm per oocyte. Some of the oocytes were fixed immediately, and the remainder were fixed after a further 1-h incubation without free-swimming sperm. As determined by light microscopy, sperm on the zona were only 3 .+-. 2% (avg. .+-. SD) acrosome-reacted at 1 min, and the incidence increased to 46 .+-. 15% during the next hour. Electron microscopy confirmed that most sperm on the zona at 1 min were acrosome-intact. A few sperm were in an early stage of the acrosome reaction. Acrosome reactions occurring on the zona during the subsequent hour appeared to be morphologically normal. Second, treatment of sperm in suspension with acid-disaggregated zonae (2 to 4 zonae/.mu.l) increased the incidence of acrosome-reacted sperm from 3 .+-. 1% to 24 .+-. 4%. We conclude that the human zona pellucida, or material intimately associated with it, can induce acrosome reactions in human sperm.