Osteogenesis and angiogenesis in regenerating bone a during transverse distraction - Quantitative evaluation using a canine model
Osteogenesis and angiogenesis in regenerating bone a during transverse distraction - Quantitative evaluation using a canine model
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DOI：
10.1097/01.blo.0000150562.24256.a4
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发表时间：
2005-04-01
影响因子：
4.2
通讯作者：
Nakamura, K
中科院分区：
文献类型：
--
作者：
Matsuyama, J;Ohnishi, I;Nakamura, K
对于某些骨缺损、弯曲畸形或小直径骨的患者，横向牵引可能比骨转移或延长更适合。然而，尚未通过建立良好对照的动物模型对横向牵张过程中的成骨或血管生成进行详细的定量评估。我们建立了犬胫骨横向牵张模型。分离矩形皮质作为牵引碎片。术后7天，每天两次施加0.5 mm牵张，持续14天。28天巩固期后，处死动物。矿化率和骨密度增加的定量评价表明，在巩固期的初始阶段，再生骨的矿化速度更快，皮质化更早。牵引区的平均血管体积比是完整对侧胫骨的三倍多。我们假设，在整个实验期间，充分保存骨髓动脉和牵引部位的稳定性可以诱导这种更快的成骨。结果表明，横向牵张技术是可行的。横向牵引技术适用于小直径骨或大面积骨缺损的患者。
For some patients with a bone defect, curved deformity, or small diameter bone, transverse distraction might be better indicated than bone transport or lengthening. However, detailed quantitative evaluation of osteogenesis or angiogenesis during transverse distraction by creating a well-controlled animal model has not been done. We established a transverse distraction model of the canine tibia. A rectangular cortex was separated for a distraction fragment. Seven days after the operation, 0.5-mm distraction was applied twice daily for 14 days. After a 28-day consolidation period, the animals were sacrificed. Quantitative evaluations of the rates of mineralization and the increases of bone mineral density indicated faster mineralization and earlier corticalization of the regenerating bone in the initial stage of the consolidation period. The average blood vessel volume ratio in the distraction area was more than three times greater than in the intact contralateral tibiae. We hypothesized that adequate preservation of the marrow arteries and stability of the distraction site throughout an experimental period could induce this faster osteogenesis. Our results indicated that the transverse distraction technique was feasible. The transverse distraction technique could be indicated for patients with small-diameter bones or with massive bone defects.