Coordination and fine motor control depend on Drosophila TRPγ.
Coordination and fine motor control depend on Drosophila TRPγ.
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DOI：
10.1038/ncomms8288
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发表时间：
2015-06-01
影响因子：
16.6
通讯作者：
Montell C
中科院分区：
文献类型：
--
作者：
Akitake B;Ren Q;Boiko N;Ni J;Sokabe T;Stockand JD;Eaton BA;Montell C
运动协调大致分为粗大运动控制和精细运动控制，两者都依赖于本体感觉器官。然而，具体在精细运动控制中起作用的通道尚不清楚。在这里，我们发现Trpγ的突变破坏了精细运动控制，同时保持了粗大运动的熟练程度。变种人在行走过程中无法协调精确的腿部运动，而且由于无法做出这项任务所需的微妙的姿势适应，在穿越大间隙时效率低下。Trpγ在本体感觉器官中表达，在神经元和神经胶质细胞中都是缝隙交叉所必需的。我们在体外表达了Trpγ，并发现其活性被膜拉伸所促进。消除Na+/Ca~(2+)交换的突变抑制Trpγ果蝇的缝隙交叉表型。我们的研究结果表明，Trpγ通过机械激活本体感觉器官，从而促进功能所需的钙离子内流，从而有助于精细运动控制。
Motor coordination is broadly divided into gross and fine motor control, both of which depend on proprioceptive organs. However, the channels that function specifically in fine motor control are unknown. Here, we show that mutations in trpγ disrupt fine motor control while leaving gross motor proficiency intact. The mutants are unable to coordinate precise leg movements during walking, and are ineffective in traversing large gaps due to an inability in making subtle postural adaptations that are requisite for this task. TRPγ is expressed in proprioceptive organs, and is required in both neurons and glia for gap crossing. We expressed TRPγ in vitro, and found that its activity is promoted by membrane stretch. A mutation eliminating the Na+/Ca2+ exchanger suppresses the gap crossing phenotype of trpγ flies. Our findings indicate that TRPγ contributes to fine motor control through mechanical activation in proprioceptive organs, thereby promoting Ca2+ influx, which is required for function.