De novo arachidonic acid synthesis in Perkinsus marinus, a protozoan parasite of the eastern oyster Crassostrea virginica
De novo arachidonic acid synthesis in Perkinsus marinus, a protozoan parasite of the eastern oyster Crassostrea virginica
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DOI：
10.1016/s0166-6851(01)00413-3
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发表时间：
2002-02-01
影响因子：
1.5
通讯作者：
Harvey, E
中科院分区：
文献类型：
--
作者：
Chu, FLE;Lund, E;Harvey, E
使用稳定同位素标记的前体（1,2 C-13-乙酸酯和棕榈酸-d(31) 酸）研究了牡蛎原生动物寄生虫 Perkinsus marinus 在体细胞阶段从头合成脂肪酸的能力。使用气相色谱/质谱和气相色谱/火焰离子化检测分析衍生自 1,2 C-13-乙酸酯和棕榈酸-d(31) 酸的脂肪酸甲酯。结果表明，体外培养的 P. marinus meronts 利用 C-13-乙酸盐合成一系列饱和和不饱和脂肪酸。饱和脂肪酸 14:0、16:0、18:0、20:0、22:0、24:0 和不饱和脂肪酸 18:1(n-9)、18:21(n-6)、210:1(n-9)、20:2(n-6)、210:21(n-9)、与前体孵育 7、14 和 21 天后，发现 210:3(n-6)、210:4(n-6) 含有 C-13。这表明meronts可以使用乙酸盐作为底物从头合成脂肪酸。培养7天和14天后，Meronts有效地将16:0-d(31)延长至18:0、20:0、22:0、24:0，但去饱和活性有限。仅检测到少量的18:1-d(29)。这表明 Meront 不能直接将外源棕榈酸或其延伸产物转化为不饱和对应物。从乙酸盐合成 20:4(n-6) 的能力特别令人感兴趣。据报道，没有寄生原生动物能够从头合成长链必需脂肪酸，例如20:4(n-6)。未来的研究将旨在确定当 Merons 与宿主相关时，观察到的体外活动是否确实反映了体内活动。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 C-13-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 C-13-acetate and palmitic-d(31) acid were analyzed using gas chromatography/mass spectrometry and gas chromatography/flame ionization detection. Results revealed that in vitro cultured P. marinus meronts utilized C-13-acetate to synthesize a range of saturated and unsaturated fatty acids. The saturated fatty acids 14:0, 16:0, 18:0, 20:0, 22:0, 24:0 and the unsaturated fatty acids, 18:1(n-9), 18:21(n-6), 210:1(n-9), 20:2(n-6), 210:21(n-9), 210:3(n-6), 210:4(n-6) were found to contain C-13, after 7, 14, and 21 days incubation with the precursor. This indicates that meronts can synthesize fatty acid de novo using acetate as a substrate. Meronts efficiently elongated 16:0-d(31) to 18:0, 20:0, 22:0, 24:0, but desaturation activity was limited, after 7 and 14 days cultivation. Only a small quantity of 18:1-d(29) was detected. This suggests that meronts cannot directly convert exogenous palmitic acid or its products of elongation to unsaturated counterparts. The ability to synthesize 20:4(n-6) from acetate is particularly interesting. No parasitic protozoan has been reported to be capable of synthesizing long chain essential fatty acids, such as 20:4(n-6) de novo. Future study will be directed to determine whether the observed in vitro activities indeed reflect the in vivo activities, when meronts are associated with the host. (C) 2002 Elsevier Science B.V. All rights reserved.