Detection and transcript expression of S-RNase gene associated with self-incompatibility in apricot (Prunus armeniaca L.)
Detection and transcript expression of S-RNase gene associated with self-incompatibility in apricot (Prunus armeniaca L.)
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DOI：
10.1007/s11033-006-0011-x
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发表时间：
2006-09-01
影响因子：
2.8
通讯作者：
Zhang, Lijie
中科院分区：
文献类型：
--
作者：
Feng, Jianrong;Chen, Xuesen;Zhang, Lijie
对自交亲和杏品种“凯蒂”和自交不亲和杏品种“新世纪”的S-RNase基因型进行了比较分析。采用等位基因特异性聚合酶链反应（AS-PCR）技术，将“凯蒂”和“新世纪”的等位基因分别命名为S8 Sc和S9 S10。S-RNase基因在两种基因型的花柱中都有表达。采用实时荧光定量RT-PCR技术，比较了“凯蒂”和“新世纪”S-RNase基因的时空表达模式。结果表明，S-RNase基因在“凯蒂”和“新世纪”中的表达存在差异。转录本丰度在关键阶段（即，自花授粉后24 h），且“新世纪”（SI）的表达量大于“凯蒂”（SC）。此外，S-RNase转录本在花柱上半部的丰度高于花柱下半部和子房。SI品种‘新世纪’异花授粉后S-RNase的表达量较高，但自花授粉和不授粉后S-RNase的表达量持续下降。
The identity and expression of S-RNase genotypes in the self-compatible (SC) apricot cultivar 'Katy' and the self-incompatible (SI) cultivar 'Xinshiji' were examined. We used allele specific polymerase chain reaction (AS-PCR) and designated the alleles in 'Katy' and 'Xinshiji' as S8Sc and S9S10, respectively. The S-RNase gene was expressed in style at the balloon stage in both genotypes. Using real-time fluorescence quantification RT-PCR technology (FQRT-PCR), spatio-temporal expression patterns of S-RNase gene between 'Katy' and 'Xinshiji' were compared. The results revealed that the expression of the S-RNase gene in 'Katy' and 'Xinshiji' were different. The transcript abundance was distinctly diverse at the key stage (i.e., at 24 h after self-pollination) in both genotypes, and was greater in 'Xinshiji' (SI) than 'Katy' (SC). In addition, the abundance of the S-RNase transcript was higher in upper-half of style than in the lower-half of style or in the ovary. In the SI cultivar 'Xinshiji', the expression of S-RNase reminded a relatively high level after cross-pollination, but it dropped continuously after self-pollination and un-pollination.