Less is more – length reduction of STR amplicons using redesigned primers
Less is more – length reduction of STR amplicons using redesigned primers
复制标题
少即是多——使用重新设计的引物减少 STR 扩增子的长度
DOI:
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发表时间:
2001
期刊:
影响因子:
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通讯作者:
M. Kleiber
中科院分区:
文献类型:
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作者:
P. Wiegand;M. Kleiber
Abstract PCR primers closely flanking the repeat region were redesigned to reduce the amplicon length of the selected STRs down to approximately 100 bp for the shorter alleles (loci HumTH01, D10S2325, DYS19 and DYS391). Highly degraded DNA (e.g. formalin-fixed tissue) and very low amounts of DNA could be more successfully typed using the new redesigned primers compared to the established sequences generating longer amplicons.
影响因子:
14.9
作者:
Edwards,MC;Clemens,PR;Tristan,M;Pizzuti,A;Gibbs,RA
通讯作者:
Gibbs,RA