Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature-dependent sex determination in pejerrey Odontesthes bonariensis
Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature-dependent sex determination in pejerrey Odontesthes bonariensis
复制标题
波纳里齿牙温度依赖性性别决定过程中Fshr和Lhr与Fshb和Lhb亚基的分子克隆及表达分析
DOI：
10.1002/mrd.21179
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发表时间：
2010
影响因子：
2.5
通讯作者：
and C.A. Strussmann
中科院分区：
文献类型：
--
作者：
T. Shinoda;L.A. Miranda;K. Okuma;R.S.Hattori;J.I. Fernandino;G. Yoshizaki;G.M. Somoza;and C.A. Strussmann
在这项研究中，我们克隆并表征了具有温度依赖性性别决定（TSD）的物种pejerreyOdontesthes bonariensis的促卵泡激素受体（Fshr）和促黄体激素受体（Lhr）cDNA，并分析了它们在性腺发育过程中在仅产生雌性（17°C，FPT）、两性（25°C，MixPT）和仅产生雄性（29°C，MPT）的温度下与Fshr和Lhr亚基的表达。pejerreyFshrcDNA全长3，069 bp，成熟蛋白由694个氨基酸组成，信号肽由22个氨基酸组成; LhrcDNA全长2，936 bp，成熟蛋白由676个氨基酸组成，信号肽由25个氨基酸组成。除了lhrin鱼在MPT，所有的基因表现出显着的增加和/或表达的峰值前性腺的组织分化，无论温度。在TSD期间，FPT组的大鼠Fshr和Lhb表达低于MPT组，但Lh表达高于MPT组; Fshr的表达模式不明确。在MixPT条件下，约半数鱼的Fshb、Lhb和LhrmRNA在TSD和性别分化过程中升高，其中雄性占55.2%。基于上述结果，建议具有高FshbLh和低Lhr值的动物代表推定的雄性。这些证据与其他研究一起表明，温度可能通过垂体（Fsh和Lhb的差异表达）向下传递到性腺（Lhr的差异表达），可能影响pejerrey TSD过程中类固醇生成的调节。摩尔Reprod. Dev. 77：521-532，2010.© 2010 Wiley利斯公司
In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerreyOdontesthes bonariensis, a species with temperature‐dependent sex determination (TSD), and analyzed their expression in relation toFshbandLhbsubunits during gonadogenesis at temperatures producing only females (17°C, FPT), both sexes (25°C, MixPT), and only males (29°C, MPT). The pejerreyFshrcDNA had 3,069 bp for a mature protein of 694 amino acids (aa) and a signal peptide of 22 aa; theLhrcDNA had 2,936 bp for a mature protein of 676 aa and a signal peptide of 25 aa. With the exception ofLhrin fish at the MPT, all genes showed significant increases and/or peaks of expression before histological differentiation of the gonads regardless of temperature. Larvae at the FPT had lowerFshbandLhbbut higherLhrexpression during the TSD period than those at the MPT; a clear pattern could not be ascertained forFshr. At the MixPT,Fshb,Lhb, andLhrmRNA increased in approximately half of the fish during TSD and sex differentiation and the sex ratio was 55.2% male. Based on the above results, it is suggested that animals with highFshbandLhband lowLhrvalues represent putative males. These evidences, together with other studies, suggest that temperature may signal through the pituitary (differential expression ofFshbandLhb) down to the gonads (differential expression ofLhr), probably affecting the regulation of steroidogenesis during the TSD process of pejerrey. Mol. Reprod. Dev. 77: 521–532, 2010. © 2010 Wiley‐Liss, Inc.