Adequate enrichment of extracellular vesicles in laboratory medicine
Adequate enrichment of extracellular vesicles in laboratory medicine
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DOI：
10.1002/inmd.20220003
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发表时间：
2023-05
期刊：
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通讯作者：
Limin Zhang;Wencui Ma;Xinyi Gan;W. Chen;Jiahui Guo;Yizhi Cui;Tong Wang
中科院分区：
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作者：
Limin Zhang;Wencui Ma;Xinyi Gan;W. Chen;Jiahui Guo;Yizhi Cui;Tong Wang
细胞外囊泡（EVs）及其临床应用已被公认为最快速增长和最有前途的领域之一。包括我们在内的许多团体都表达了他们对出于不同目的分离“纯”EV的想法的担忧，因为迄今为止还没有特定的标志物可以检查确切的EV纯度。通常，主要的仪器需求和EV产率损失是获得具有更高纯度的EV的主要补偿。因此，特别是在基于生物标志物的临床试验中，为获得更纯的EV而不断努力是值得讨论的。我们在这里提出了另一种哲学来淡化纯度的重要性，但强调临床实验室医学中测量通过良好控制的分离方法获得的EV的重要性。因此，EV被富集到足够的程度以满足某些临床应用，例如疾病诊断和/或预后。在这篇综述中，我们将通过EV定义、标志物和污染物、EV分离方法的进展以及使用纯度较低但易于操作的EV分离方法从临床研究中获得的结论来证明充分富集的合理性。
Extracellular vesicles (EVs) and their clinical applications have been recognized as one of the most fast‐growing and promising fields. Numerous groups including us have expressed their concerns on the idea of isolating “pure” EVs for different purposes, as there are no specific markers to date that can examine the exact EV purity. Typically, major instrument need and EV yielding loss are primary compensations of getting EVs with higher purity. As such, especially in biomarker‐based clinical tests, ceaseless efforts for obtaining more pure EV are worth of discussion. We here propose alternative philosophy to tone down the importance of purity, but to emphasize the significance in clinical laboratory medicine to measure EVs obtained by well‐controlled isolation methods. As a result, EVs are enriched to the adequate degree to meet certain clinical applications such as disease diagnosis and/or prognosis. In this review, we will justify the adequate enrichment rationale with advances of EV definitions, markers and contaminants, EV isolation methods, as well as the conclusions acquired from clinical research using less pure but ease‐to‐operate EV isolation methods.