NON-INVASIVE MEASUREMENT OF GLUCOSE AND PYRUVATE UPTAKE BY INDIVIDUAL HUMAN OOCYTES AND PREIMPLANTATION EMBRYOS
NON-INVASIVE MEASUREMENT OF GLUCOSE AND PYRUVATE UPTAKE BY INDIVIDUAL HUMAN OOCYTES AND PREIMPLANTATION EMBRYOS
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DOI：
10.1093/oxfordjournals.humrep.a136869
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发表时间：
1989-02-01
影响因子：
6.1
通讯作者：
LEESE, HJ
中科院分区：
文献类型：
--
作者：
HARDY, K;HOOPER, MAK;LEESE, HJ
用超微荧光法对73个人卵母细胞和植入前胚胎的丙酮酸和葡萄糖摄取量进行了非侵入性测量，以分析培养液微滴中底物水平的变化。从第1天(未受精的卵母细胞)到第6天(第0天=受精日)，连续培养2天(未移植的“备用”胚胎)，测量两种底物的吸收。在此条件下，58%(25/43)的Dauy 1有两个原核的受精胚胎在第6天发育到囊胚。这些胚胎的丙酮酸摄取从apprx开始增加。在2.5天到4.5天之间，每小时最高可达40 pmol/胚胎。同样，.apprx的葡萄糖摄取量也有所增加。2.5~4.5天为8~14pmoL/胚胎/h，第5天囊胚期进一步增加至24pmoL/胚胎/h。受精卵的丙酮酸摄取率为4.5，但在囊胚期第5天进一步增加到24pmoL/胚胎/h。卵裂期受精胚胎的丙酮酸摄取显着低于发育到囊胚期的受精胚胎。多精子胚胎和孤雌胚胎以及未受精的卵母细胞在后期对丙酮酸的摄取量也较低。未受精的卵母细胞和异常胚胎的葡萄糖摄取在5.5d的囊胚期仍未达到受精胚胎的水平。胚胎移植前对丙酮酸摄取的非侵入性测量可能为选择能够发育到囊胚期的可存活胚胎提供一个有价值的功能标准。
Pyruvate and glucose uptake by 73 individual human oocytes and preimplantation embryos was measured non-invasively, using an ultramicrofluorescence assay to analyse changes in substrate levels in microdroplets of culture medium. The uptake of both substrates was measured over successive daily incubations between days 1 (unfertilized oocytes) for 2 (''spare'' embryos which were not transferred) and day 6 (day 0 = day of insemination). Under these conditions, 58% (25/43) of fertilized embryos with two pronuclei on dauy 1 developed to the blastocyst stage by day 6. The pyruvate uptake of these embryos increased from .apprx. 28 to a maximum of 40 pmol/embryo/h between days 2.5 and 4.5. Similarly, glucose uptake increased from .apprx. 8 to 14 pmol/embryo/h between days 2.5 and 4.5, but then increased further to 24 pmol/embryo/h on day 5 at the blastocyst stage. The pyruvate uptake of fertilized 4.5, but then increased further to 24 pmol/embryo/h on day 5 at the blastocyst stage. The pyruvate uptake of fertilized embryos which arrested at cleavage stages was significantly lower than for those which developed to the blastocyst stage. Polyspermic and parthenogenetic embryos, and unfertilized oocytes also had lower pyruvate uptakes at later stages. The glucose uptake of unfertilized oocytes and abnormal embryos never reached the level of fertilized embryos at the blastocyst stage on day 5.5. Non-invasive measurement of pyruvate uptake before embryo transfer may provide a valuable functional criterion for the selection of viable embryos capable of developing to the blastocyst stage.