Metabolic pathway characterization from transient response data obtained in situ: parameter estimation in S-system models.
Metabolic pathway characterization from transient response data obtained in situ: parameter estimation in S-system models.
复制标题
原位获得的瞬态响应数据的代谢途径表征：S 系统模型中的参数估计。
DOI：
10.1006/jtbi.1993.1078
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发表时间：
1993
影响因子：
2
通讯作者：
M. Cascante
中科院分区：
文献类型：
--
作者：
A. Sorribas;S. Samitier;E. Canela;M. Cascante
体内代谢物和通量的实际值可以通过一些实验技术来测量，它们为在原位评估代谢途径的性质提供了重要信息。在本文中，我们提出了一种适当利用这些信息的策略。该方法允许对整个系统的一组参数进行估计，从而可以构建一个有用的模型，用于描述其组件和系统特性，并预测其在新条件下的行为。提供了一个模拟的参考途径，以验证该方法并显示其在代谢研究中的有效性。
The actual values of internal metabolites and fluxes can be measured by a number of experimental techniques and they provide important information for evaluating the properties of a metabolic pathway in situ. In this paper we propose a strategy to properly exploit this information. The suggested approach permits estimation of a set of parameters on the whole system so that a useful model can be constructed and used to describe its components and systemic properties and to predict its behavior under new conditions. A simulated reference pathway is provided to validate this method and to show its utility in metabolic studies.