The effect of genetic background on behavioral manifestation of Grid2Lc mutation
The effect of genetic background on behavioral manifestation of Grid2Lc mutation
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DOI：
10.1016/j.bbr.2014.06.023
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发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Vozeh, Frantisek
中科院分区：
文献类型：
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作者：
Cendelin, Jan;Tuma, Jan;Vozeh, Frantisek
突变小鼠是遗传性疾病的常用模型。然而，这些小鼠具有原始菌株的表型特征，这可能会干扰感兴趣突变的表现。Lurcher小鼠代表了一种由GRID2(LC)突变引起的橄榄小脑变性模型。摇摇晃晃的人表现出共济失调以及各种认知和行为异常。最常用的Lurcher小鼠品系是B6CBA和C3H，但没有关于遗传背景在GRID2(LC)表现中的作用的信息。本工作的目的是比较B6CBA和C3HLurcher突变小鼠和野生型小鼠在Morris水迷宫中的空间航行、在开阔场地的自发活动以及在水平绳索、斜梯和轮杆上的运动技能。研究表明，这两种品系的Lurcher小鼠的运动技能和水迷宫表现都受到了损害。C3HLurcher和OH野生型小鼠在水迷宫任务中的表现都比B6CBA小鼠差。在旷场实验中，C3H小鼠表现出较高的活动度和较低的趋毛性。研究表明，遗传背景可以改变勒彻突变的表现。在这种情况下，B6CBA Lurcher小鼠模型在研究小脑变性的行为方面可能比OH Lurcher小鼠更有效，因为它们没有将与GRID2(LC)突变相关的异常与菌株特有的问题结合在一起。(C)2014爱思唯尔B.V.保留所有权利。
Mutant mice are commonly used models of hereditary diseases. Nevertheless, these mice have phenotypic traits of the original strain, which could interfere with the manifestation of the mutation of interest. Lurcher mice represent a model of olivocerebellar degeneration, which is caused by the Grid2(Lc) mutation. Lurchers show ataxia and various cognitive and behavioral abnormalities. The most commonly used strains of Lurcher mice are B6CBA and C3H, but there is no information about the role of genetic background on the Grid2(Lc) manifestation. The aim of this work was to compare spatial navigation in the Morris water maze, spontaneous activity in the open field and motor skills on the horizontal wire, slanted ladder and rotarod in B6CBA and C3H Lurcher mutant and wild type mice. The study showed impaired motor skills and water maze performance in both strains of Lurcher mice. Both C3H Lurcher and OH wild type mice had poorer performances in the water maze task than their B6CBA counterparts. In the open field test, C3H mice showed higher activity and lower thigmotaxis. The study showed that genetic backgrounds can modify manifestations Of the Lurcher mutation. In this case, B6CBA Lurcher mice models probably have more validity when studying the behavioral aspects of cerebellar degeneration than OH Lurcher mice, since they do not combine abnormalities related to the Grid2(Lc) mutation with strain-specific problems. (C) 2014 Elsevier B.V. All rights reserved.