An isolate of Aspergillus flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase gene
An isolate of Aspergillus flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase gene
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DOI：
10.1007/s00253-004-1670-y
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发表时间：
2004-09-01
影响因子：
5
通讯作者：
Cotty, PJ
中科院分区：
文献类型：
--
作者：
Ehrlich, KC;Cotty, PJ
某些食品和饲料被高毒性和致癌性的曲霉菌毒素家族黄曲霉毒素污染，会给农民带来严重的经济负担。作为减少黄曲霉毒素污染的策略之一，非产黄曲霉毒素A。黄曲霉分离物AF 36目前被应用于农业领域以竞争性地排除产生黄曲霉毒素的曲霉属物种。我们现在表明，聚酮合酶基因（PKSA）所需的黄曲霉毒素的生物合成在AF36，并在其他成员的相同的营养兼容性组，具有核苷酸多态性附近的编码序列的开始。这种核苷酸的改变将提前终止密码子引入编码序列，从而防止酶的产生和黄曲霉毒素的积累。
Contamination of certain foods and feeds with the highly toxic and carcinogenic family of Aspergillus mycotoxins, the aflatoxins, can place a severe economic burden on farmers. As one strategy to reduce aflatoxin contamination, the non-aflatoxin-producing A. flavus isolate AF36 is currently being applied to agricultural fields to competitively exclude aflatoxin-producing Aspergillus species. We now show that the polyketide synthase gene (pksA) required for aflatoxin biosynthesis in AF36, and in other members of the same vegetative compatibility group, possesses a nucleotide polymorphism near the beginning of the coding sequence. This nucleotide change introduces a premature stop codon into the coding sequence, thereby preventing enzyme production and aflatoxin accumulation.