Autoinducer 2 (AI-2) Production by Nontypeable Haemophilus influenzae 86-028NP Promotes Expression of a Predicted Glycosyltransferase That Is a Determinant of Biofilm Maturation, Prevention of Dispersal, and Persistence In Vivo.
Autoinducer 2 (AI-2) Production by Nontypeable Haemophilus influenzae 86-028NP Promotes Expression of a Predicted Glycosyltransferase That Is a Determinant of Biofilm Maturation, Prevention of Dispersal, and Persistence In Vivo.
复制标题
不可分型流感嗜血杆菌 86-028NP 产生的自诱导子 2 (AI-2) 可促进预测的糖基转移酶的表达，该酶是生物膜成熟、防止扩散和体内持久性的决定因素。
DOI：
10.1128/iai.00506-18
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发表时间：
2018
影响因子：
3.1
通讯作者：
Swords,WEdward
中科院分区：
文献类型：
--
作者：
Pang,Bing;Armbruster,ChelsieE;Foster,Gayle;Learman,BrianS;Gandhi,Uma;Swords,WEdward
Nontypeable Haemophilus influenzae (NTHi) is an extremely common human pathobiont that persists on the airway mucosal surface within biofilm communities, and our previous work has shown that NTHibiofilm maturation is coordinated by the production and uptake of autoinducer 2 (AI-2) quorum signals. To directly test roles for AI-2 in maturation and maintenance of NTHibiofilms, we generated an NTHi86-028NP mutant in whichluxStranscription was under the control of thexylApromoter (NTHi86-028NPluxS xylA::luxS), rendering AI-2 production inducible by xylose. Comparison of biofilms under inducing and noninducing conditions revealed a biofilm defect in the absence of xylose, whereas biofilm maturation increased following xylose induction. The removal of xylose resulted in the interruption ofluxSexpression and biofilm dispersal. Measurement ofluxStranscript levels by real-time reverse transcription-PCR (RT-PCR) showed thatluxSexpression peaked as biofilms matured and waned before dispersal. Transcript profiling revealed significant changes following the induction ofluxS, including increased transcript levels for a predicted family 8 glycosyltransferase (NTHI1750; designatedgstA); this result was confirmed by real-time RT-PCR. An isogenic NTHi86-028NPgstAmutant had a biofilm defect, including decreased levels of sialylated matrix and significantly altered biofilm structure. In experimental chinchilla infections, we observed a significant decrease in the number of bacteria in the biofilm population (but not in effusions) for NTHi86-028NPgstAcompared to the parental strain. Therefore, we conclude that AI-2 promotes NTHibiofilm maturation and the maintenance of biofilm integrity, due at least in part to the expression of a probable glycosyltransferase that is potentially involved in the synthesis of the biofilm matrix.
