BACTERIAL METABOLISM OF 2-METHYLALANINE
BACTERIAL METABOLISM OF 2-METHYLALANINE
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DOI：
10.1128/jb.88.5.1296-1303.1964
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发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
LARSON, AD
中科院分区：
文献类型：
--
作者：
AASLESTAD, HG;LARSON, AD
Aaslestad, H. G.（路易斯安那州立大学，巴吞鲁日）和 A. D. Larson。 2-甲基丙氨酸的细菌代谢。 J.细菌学。88：1296–1303。 1964 年——人们发现从土壤中分离出的一种细菌可以通过适应性酶系统将 2-甲基丙氨酸氧化至理论值的 65%。全细胞测压实验表明丙酮而非异丙胺是中间体。当丙酮酸添加到反应混合物中时，细胞提取物从 2-甲基丙氨酸产生二氧化碳。透析刺激 2-甲基丙氨酸代谢系统，提供磷酸吡哆醛和丙酮酸。对这种磷酸吡哆醛依赖性反应的化学计量研究表明丙酮和二氧化碳是主要产物。 2-甲基丙氨酸形成二氧化碳似乎依赖于氨基向磷酸吡哆醛的转移。发现其他 2-甲基氨基酸被该系统脱羧，因此表明缺乏绝对特异性。
Aaslestad, H. G. (Louisiana State University, Baton Rouge),and A. D. Larson. Bacterial metabolism of 2-methylalanine. J. Bacteriol.88:1296–1303. 1964.—A bacterium isolated from soil was found to oxidize 2-methylalanine to 65% of the theoretical value by an adaptive enzyme system. Manometric experiments with whole cells indicated that acetone but not isopropylamine was an intermediate. Cellular extracts produced carbon dioxide from 2-methylalanine when pyruvate was added to the reaction mixture. Dialysis stimulated the 2-methylalanine metabolizing system, providing pyridoxal phosphate and pyruvate were supplied. A study of the stoichiometry of this pyridoxal phosphate-dependent reaction indicated that acetone and carbon dioxide were principal products. The formation of carbon dioxide from 2-methylalanine seemed dependent upon transfer of the amino group to pyridoxal phosphate. Other 2-methyl amino acids were found to be decarboxylated by this system, thus indicating a lack of absolute specificity.