BK VIRUS SPECIFIC IGM RESPONSES IN CORD SERA, YOUNG-CHILDREN AND HEALTHY-ADULTS DETECTED BY RIA
BK VIRUS SPECIFIC IGM RESPONSES IN CORD SERA, YOUNG-CHILDREN AND HEALTHY-ADULTS DETECTED BY RIA
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DOI：
10.1007/bf01311159
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
FIELD, AM
中科院分区：
文献类型：
--
作者：
BROWN, DWG;GARDNER, SD;FIELD, AM
本文报道了BK病毒(BKV)特异性IgM的IgM捕获固相放射免疫分析法(MACRIA)。本试验检测BVK特异性IgM比血凝抑制和免疫EM法更敏感。使用这种特异的检测方法，可以方便地检测大量血清，以便更全面地研究BKV特异性IgM在不同人群中的分布。在伦敦献血员血清中检出BKV特异性IgM，在2~11岁儿科住院儿童血清中检出24/114份，在2~5岁儿童血清中检出14/79份，以调查抗链球菌素0效价。为调查BK病毒经胎盘传播，在404份脐带血清中未检测到BKV特异性IgM。
An IgM capture solid-phase radioimmunoassay (MACRIA) for BK virus (BKV) specific IgM is described. This test is more sensitive in detecting BVK specific IgM than hemagglutination inhibition and immune EM with serum fractions from sucrose density gradients. The use of this specific assay allowed large numbers of sera to be examined with ease so that the distribution of BKV specific IgM in different populations could be studied more fully. BKV specific IgM was detected in 11/300 sera from London [UK] blood donors, in 24/114 sera from children aged between 2-11 yr admitted to a pediatric unit and 14/79 sera taken from children aged between 2-5 yr for the investigation of anti-streptolysin 0 titers. BKV specific IgM was not detected in 404 cord sera examined to investigate the transplacental transmission of BK virus.