Selective isopeptide bond formation:: coupling ubiquitin67-76 with histone 2A114-128 by use of the transfer active ester condensation technique
Selective isopeptide bond formation:: coupling ubiquitin67-76 with histone 2A114-128 by use of the transfer active ester condensation technique
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DOI：
10.1034/j.1399-3011.1999.00075.x
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发表时间：
1999-06-01
期刊：
影响因子：
--
通讯作者：
Ramage, R
中科院分区：
文献类型：
--
作者：
Wang, P;Layfield, R;Ramage, R
通过在泛素(67-76)的C末端与组蛋白2A(114-128)中赖氨酸119的E-氨基之间选择性地形成一个异肽键，合成了一个多肽--泛素(67-76)-组蛋白2A(114-128)片段(UBH_2AF)，它在第118、119、125和127位分别含有4个赖氨酸残基。将转移活性酯缩合技术与氨基保护基团相结合，成功地将转移活性酯缩合技术应用于reaction.Leu(67)-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly(76)/Val(114)-Leu-Leu-Pro-Lys(NH2)-Lys(119)-Thr-Glu-Ser-His-His-Lys(NH2)-Ala-Lys(NH2)-Gly(128)UBH_2AF偶联多肽片段中。
A peptide, ubiquitin(67-76)-histone 2A(114-128) fragment (UBH2AF), was synthesized by selective formation of an isopeptide bond between the C-terminus of ubiquitin(67-76) and the E-amino group of lysine-119 in histone 2A(114-128) which contained 4 lysine residues at positions 118, 119, 125 and 127, respectively. The transfer active ester condensation technique, together with the Tnm amine protecting group, were used successfully in the peptide segment coupling reaction.Leu(67)-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly(76) / Val(114)-Leu-Leu-Pro-Lys(NH2)-Lys(119)-Thr-Glu-Ser-His-His-Lys(NH2)-Ala-Lys(NH2)-Gly(128) UBH2AF.