Polygraphic Recording Procedure for Measuring Sleep in Mice
Polygraphic Recording Procedure for Measuring Sleep in Mice
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DOI：
10.3791/53678
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发表时间：
2016-01-01
影响因子：
1.2
通讯作者：
Lazarus, Michael
中科院分区：
文献类型：
--
作者：
Oishi, Yo;Takata, Yohko;Lazarus, Michael
记录小动物（如小鼠和大鼠）的硬膜外脑电图（EEG）和肌电图（EMG）是研究睡眠-觉醒调节的稳态动力学和回路的关键。在许多实验室中，使用基于电缆的睡眠记录系统来监测自由行为小鼠的EEG和EMG，并结合计算机软件，以基于EEG数据的功率谱分析来自动评分警惕状态。本文详细描述了该系统。在一侧大脑半球的额叶皮质区和顶叶区植入钢制螺钉，用于监测EEG信号。此外，EMG活动通过在两个颈部肌肉中的导线的双侧放置来监测。非快速眼动（Non-REM; NREM）睡眠的特征在于在EEG中具有低于4 Hz的δ活动的大而慢的脑电波，而在从NREM到REM睡眠的过渡处可以观察到在6和10 Hz之间的θ范围中从低频δ活动到快速低电压EEG的转变。相比之下，清醒是通过脑电图中的低到中等电压的脑电波和显著的肌电图活动来识别的。
Recording of the epidural electroencephalogram (EEG) and electromyogram (EMG) in small animals, like mice and rats, has been pivotal to study the homeodynamics and circuitry of sleep-wake regulation. In many laboratories, a cable-based sleep recording system is used to monitor the EEG and EMG in freely behaving mice in combination with computer software for automatic scoring of the vigilance states on the basis of power spectrum analysis of EEG data. A description of this system is detailed herein. Steel screws are implanted over the frontal cortical area and the parietal area of 1 hemisphere for monitoring EEG signals. In addition, EMG activity is monitored by the bilateral placement of wires in both neck muscles. Non-rapid eye movement (Non-REM; NREM) sleep is characterized by large, slow brain waves with delta activity below 4 Hz in the EEG, whereas a shift from low-frequency delta activity to a rapid low-voltage EEG in the theta range between 6 and 10 Hz can be observed at the transition from NREM to REM sleep. By contrast, wakefulness is identified by low-to moderate-voltage brain waves in the EEG trace and significant EMG activity.